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Introduction
The first German team to participate in iGEM ever is back again, providing you with an excellent set of fusion proteins for modular use as [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Freiburg BioBricks]. This year we are crafting a synthetic receptor kit "made in Black Forest", using DNA-Origami as programmable input device and the complementation of split-fluorophores and -enzymes as readable output.
An [http://parts.mit.edu/igem07/index.php/Freiburg07/report_fusion_parts extension of the standard] BioBrick Pre- and Suffix, developed by iGEM-Team Freiburg 2007, allows in-frame-cloning of proteins without generation of a stop-codon at the "scar", so that we can fuse practically any number of proteins and/or peptides - as you can see at our cloning strategy table. Of course we want to share this possibility with future iGEM-Teams and have added a new, fully compatible [http://partsregistry.org/Part:BBa_K157000 plasmid] to the registry.
Abstract
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