Team:Hawaii/Protocols
From 2008.igem.org
Projects | Events | Resources | ||
---|---|---|---|---|
Sponsors | Experiments | Milestones | Protocols | |
Notebook (t) | Meetings (t) |
List of Protocols
This is a list of tried and true protocols used by our team.
E. coli
- Cryopreserve cells
- Making competent cells
- Transformation of E. coli
- Alkaline lysis / plasmid isolation
Synechocystis sp. PCC6803
Media
- LB
- LB + amp100
- LB + kan20 + amp 50
- LB + sp100
- LB + sp100 + sm50
- BG-11 + 5% LB
- BG-11
- BG-11 + sp2.5 + sm2.5
- Liquid growth media
BioBrick
- [http://openwetware.org/wiki/BioBricks_construction_tutorial BioBrick Construction Tutorial]
- [http://openwetware.org/wiki/Silver:_BB_Strategy Silver:BioBrick Strategy]
- [http://openwetware.org/wiki/Designing_primers#BioBrick_primers Designing BioBrick Primers]
- [http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication BioBrick Part Fabrication]
[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]