Team:Chiba/Calendar-Home/31 August 2008

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30 August 2008 <|> 1 September 2008

Contents

1 Laboratory work

Laboratory work

Team:Input

no work

Team:Communication

Transformation

Competent Cells : XL10Gold

[http://partsregistry.org/Part:BBa_K084007 BBa_K084007](Plac+RBS+LasI)
[http://partsregistry.org/Part:BBa_K084008 BBa_K084008](Plac+RBS+RhlI)

---> We saved these plates with a refrigerator.

Digestion

[http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
[http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)
[http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)

Sample No.	1~3	4
Sample DNA(μL)	12	10
PstⅠ(μL)	0.2	0.2
XbaⅠ(μL)	0.2	-
SpeⅠ(μL)	-	0.4
Buffer 2(μL)	-	1.5
Buffer 3(μL)	2	-
BSA(μL)	2	1.5
dH₂O(μL)	3.6	1.4
TOTAL(μL)	20	15

--->(1/9)Gel Check

(30/8)--->Mini prep

--->Digestion test

[http://partsregistry.org/Part:BBa_K084008 BBa_K084008] Sample No.2~5
[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1),(2)

Sample	Single Digesiton 2~5	Double Digestion 2~5	Single Digestion T9002(1)	Single Digesiton T9002(2)	Double Digestion T9002(1)(2)
Sample DNA(μL)	1	3	3	1	3
XbaI(μL)	0.1	0.1	0.1	0.1	0.1
SpeI(μL)	-	0.1	-	-	0.1
Buffer 2(μL)	0.9	0.8	0.9	0.9	0.8
BSA(μL)	1	1	1	1	1
dH₂O(μL)	7	5	5	7	5
TOTAL(μL)	10	10	10	10	10

--->Gel Check

Sample No.	[http://partsregistry.org/Part:BBa_K084008 BBa_K084008] Sample No.2~5, [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1)(2)	Single & Double Digestion
Sample DNA(μL)	1	10
Loading Dye(μL)	1	2
dH₂O(μL)	4	-
TOTAL(μL)	6	12

From Left

Single Digestion [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] 2~5
Single Digestion [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1),(2)
Double Digestion [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] 2~5
Double Digestion [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1),(2)
[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1),(2)

Team:Output

[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]①
[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]②

Sample No.	①	②
DNA	10	10
SpeⅠ	1	0
PstⅠ	1	1
XbaⅠ	0	1
BSA(x100)	0.5	0.5
Buffer2	5	0
Buffer3	0	5
dH₂O	28.5	28.5
TOTAL	50μl	50μl

-->left for 3 hours at 37 °C.

-->①20μl refine

-->②20μl refine

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