Alberta NINT/25 June 2008
From 2008.igem.org
lab work (JD):
Decided to try AGAIN to recreate K102007 but using older versions of K102005/B+N that seemed to be cut better when looking at gels and ligating them to the newly annealed TA0/B+N (a combination I still haven't tried) Created K102007(1): K102005/B+N from June 18, and TA0/B+N newly annealed Created K102007(2): K102005/B+N from June 12 and TA0/B+N newly annealed Created K102006: K102003/B+N and TA0/B+N newly annealed Transformed XL1-B cells with the ligations and negative controls Plated cells