Team:Chiba/Calendar-Home/20 August 2008

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Laboratory work

Team:Input

PCR

[http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)(1)
[http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2008)(2)
[http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)(3)
[http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2008)(4)
[http://partsregistry.org/Part:BBa_E0612 BBa_E0612](2008)(5)

Sample No.	1	2	3	4	5
DNA tamplate(μL)	1	1	1	1	1
FW primer(μL)	5	5	5	5	5
VR primer(μL)	5	5	5	5	5
dNTPmix(μL)	10	10	10	10	10
thermo pol buffer(μL)	10	10	10	10	10
DNA pol(VENT)(μL)	1	1	1	1	1
dH₂O(μL)	68	68	68	68	68
TOTAL(μL)	100	100	100	100	100

-->Gel Check

>

Sample No.	1	2	3	4	5
Sample DNA	1	1	1	1	1
loading dye(μL)	1	1	1	1	1
dH₂O(μL)	4	4	4	4	4
Total(μL)	6	6	6	6	6

-->1,2,3,4,5:none

Transformation

[http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2007)
[http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2006)
[http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2007)
[http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2006)
[http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)
[http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)-->only one colony

Team:Communication

(18/8)--->Mini prep

[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2007)
[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2006)
[http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007)
[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007)
[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006)
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)

--->Gel Check

Sample DNA	3
Loading Dye	2
dH₂O	7
TOTAL	12μL

From left;

[http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007) -> None
[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007) -> None
[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006) -> None

Sample DNA	10
Loading Dye	4
dH₂O	10
TOTAL	24μL

From left;

[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007) -> None
[http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006) -> OK
[http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007) -> None
[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2007) -> OK
[http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2006) -> OK

Sample DNA	3
Loading Dye	2
dH₂O	7
TOTAL	12μL

From left;

[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -> OK
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) -> OK
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) -> OK
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) -> OK

--->Digestion test

[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)

Sample No	1	2	3	4	5	6	7	8
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)	3	-	-	-	3	-	-	-
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)	-	3	-	-	-	3	-	-
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)	-	-	3	-	-	-	3	-
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)	-	-	-	3	-	-	-	3
EcoRI	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1
PstI	-	-	-	-	0.1	0.1	0.1	0.1
Buffer EcoRI	1	1	1	1	1	1	1	1
BSA	1	1	1	1	1	1	1	1
dH₂O	4.9	4.9	4.9	4.9	4.8	4.8	4.8	4.8
TOTAL	10μL	10μL	10μL	10μL	10μL	10μL	10μL	10μL

--->Gel Check

Sample DNA	3
Loading Dye	2
dH₂O	7
TOTAL	12μL

From left;

(Single Digestion 1~4)

[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) -> OK
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -> OK
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) -> OK
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) -> OK

(Double Digestion 1~4)

[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) -> OK
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -> OK
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) -> OK
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) -> OK

Team:Output

Transformation

[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](2007)-->colony
[http://partsregistry.org/Part:BBa_I712019 BBa_I712019](2008)-->no colony
[http://partsregistry.org/Part:BBa_J33202 BBa_J33202](2007)-->colony
[http://partsregistry.org/Part:BBa_E2030 BBa_E2030](2007)-->not so good
YFP(P-GEX)-->colony(Amp)
[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)-->colony
[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](2007)-->colony

Team:Chiba/Calendar-Home/20 August 2008

From 2008.igem.org

Contents

Laboratory work

Team:Input

Team:Communication

Team:Output