Team:University of Chicago/Notebook/Norayucel

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July 18, 2008

1. Diluted 1ng/microliter pGreen plasmid to 10pg/microliter with 100X dilution 2. 1microliter of plasmid to 50 microliters competent cells 3. Will grow up 50microliters of TOP10 untransformed as a control 4. Put on ice at 2:03 5. Took off ice at 2:34 6. Incubated for 1minute at 42C 7. Added 250microliters SOC media (prepared at 1:30) 8. Start incubated at 37C at 2:36 9. Take out at 3:40pm 10. Streaked 20microliters onto Amp. Plates 11. Dan will come in Saturday morning to pick up the plates and count colonies.

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