Team:Hawaii/Notebook/2008-10-27
From 2008.igem.org
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Things we did today
Wetlab work
Triparental conjugation
- Grace
- Checked on triparental conjugation cultures from 10/13. No growth was observed in any of the cultures.
- E. coli were grown at too low antibiotic concentration (sp2.5). Cells probably lost plasmid during growth, before conjugation. Will repeat triparental conjugation on Wednesday.
Plasmid prep of BBpRL1383a
- Grace
- Used Qiaprep Spin Miniprep Kit to prep the rest of the BBpRL1383a liquid cultures.
Cryostocked BBpRL1383a
- Grace
- Cryostocked 10/22 culture of DH5α w/ BBpRL1383a
Restriction digest
- Grace
- 6 hour XbaI/SpeI digest (for pretty picture):
- nir, nir+rbs, slr, pilA, slr+GFPf, GFPf
- Overnight HindIII/BamHI digest:
- J33207
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]