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Team:Edinburgh/Protocols
From 2008.igem.org
Protocols
As part of our project we developed and tested a number of procedures to make BioBrick production easier, more reliable and less hassle. These are the following:
- Use of Edinbrick1 in preparation of BioBricks from PCR products
- Site-directed mutagenesis using the MABEL protocol
- BABEL: BioBrick assembly with blunt-ended ligation
- Bacillobricks: introduction of BioBricks into Bacillus subtilis
