From 2008.igem.org
Under Construction...
Current issues:
Templates can't be stored within the Team namespace!
They must either be stored under the Template namespace or the User namespace.
A mail has been sent to Meagan of the IGEM team to see whether it is allowed to have a team folder under the
Template namespace.
Templates
General
Navigation Bar
Usage: {{:Team:KULeuven/Tools/Navigation_Bar}}
Lab Templates
Usage:
{{:Team:KULeuven/Tools/PCR
|tanneal=55
|telong=2'30"
|ncycles=30
|buffer=5x 10μL
|mgcl=10μM 0μL
|dntp=10μM 1μL
|fwprimername=6 LacZ-Fw
|fwprimerconc=10μM 2.5μL
|rvprimername=7 LacZ-Rv
|rvprimerconc=10μM 2.5μL
|water=34μL
|polymerase=Taq 0.5μL
|dna=0.5μL of E. coli DNA extract
|size=952bp
|success=YES/NO
|image=pcrproducts_GE.jpg
|lane=3+4
}}
Result:
| PCR:
|
| PCR Settings
|
| Annealing (°C)
|
| 55°
|
| Time Elongation
|
| 2'30"
|
| Number of Cycles
|
| 30
|
|
| Buffer (5x)
| 5x 10μL
|
| MgCl2 10 µM
| 10μM 0μL
|
| dNTP 10 µM
| 10μM 1μL
|
| Oligo F 10 µM
| 6 LacZ-Fw
| 10μM 2.5μL
|
| Oligo R 10 µM
| 7 LacZ-Rv
| 10μM 2.5μL
|
| Water
| 34μL
|
| Polymerase
| Taq 0.5μL
|
| DNA
| 0.5μL of E. coli DNA extract
|
|
| Expected Size
|
| 952bp
|
| Success
|
| YES/NO
|
| Image (click to enlarge)
|
| 30px
|
| Lane (0=ladder)
|
| 3+4
|
|
Digestion sheet
Usage: ...
Ligation sheet
Usage: ...
CSS
Calendar CSS file required for notebook.
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