Wisconsin: Lignin Project/2 July 2008

Revision as of 20:16, 31 July 2008 by Cburns (Talk | contribs)

Team Sorbitol

The pBAD30 was harvested using a Qiagen Miniprep kit and digested as described before.

We were able to extract enough plasmid DNA to perform a ligation.

We then set up a ligation with the following rations of vector to insert:

V:I

3 1

1 1

1 3

1 5

1 0

0 1

alowed to ligate at 4C overnight.