"
Edinburgh/18 July 2008
From 2008.igem.org
Week 5
Friday 18 July 08
- Made pSB1A2+appY Maxiprep *X3*. (AM)
- Purified PCR products for cenA, cenB, cenC and cex (P16, P17, P18, P19) run on Gel 16. No visible bands. (AM)
- Second attempt at PCR for cenA, cenB, cenC and cex (P20, P21, P22, P23). Annealing 55°C, extension 85s; did not include glycerol. Ran unpurified PCR products on Gel 17 - no bands apart from thick bands in the <0.5kb region = primers. Hence, PCR was unsuccessful. (AM)
