Team:Hawaii/Notebook/2008-08-22
From 2008.igem.org
Projects | Events | Resources | ||
---|---|---|---|---|
Sponsors | Experiments | Milestones | Protocols | |
Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Verification of transformants
- Grace
Construct | Colony forming units |
---|---|
nir+rbs | 187 |
plac+rbs | 0 |
rbs+GFP | 1 |
GFP+tt | 0 |
- Colony PCR of transformants
- Ran PCR reaction on 2% agarose gel
Plasmid prep of J33207
- Grace
Overnight RE digest
- Grace
- BB-pRL1383a and J33207 with XbaI and PstI
- pSB1A2 with EcoRI and PstI
- GFP, GFPf, nir with EcoRI and SpeI
Drylab Work
Name of Task
- name of person/people who performed the task
- Summary of task and what was done. Link to experiment for detailed notes if necessary.
- e.g. read through papers, worked on proposal, etc.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]