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Edinburgh/29 July 2008
From 2008.igem.org
Week 7
Tuesday 29 July 08
- Transformation of L20 and L21 failed.
- Transformation of glgC-mut1,2 ligation product, made plate 67 & 68 (Yan)
- Cut out putative cenA and cex (both ~1.5kb) fragments from gel 27 (repeat of Gel 26 but stained with SYBRsafe). Purified DNA from gel fragments: F1 (putative cenA) and F2 (putative cex). (AM)
- Digests of M49 to M54 (pSB1A2+rbs+crtI) with EcoRI alone. This should generate a single 4 kb band (doing an EcoRI/PstI double digest would give a vector and insert band both around 2 kb, which would probably run at the same place, making it hard to tell whether there was an insert). These were run on Gel 28. All showed a single band at the expected place. (CF)
- Minipreps of pSB1A2+pZntA (M55 to M60) from L23 (Yan, Nimisha)
- Minipreps of pSB1A2+rbs+crtE (M61 to M66) from L22 (Yan, Nimisha)
- Double digests of M55 to M60 and M61 to M66 with EcoRI/PstI. This should generate the following bands: ~2.2 kb for pZntA (M55 to M60), ~1.1 kb for rbs+crtE (M61 to M66), and ~2 kb for the pSB1A2 vector. These were run on Gel 29. M55 to M60 unsuccessful; M61 to M66 showed a band at the expected location for rbs+crtE but seemed to be indicate that L22 was still ligated into BABEL2 instead of pSB1A2. (Yan & AM)
