Edinburgh/5 August 2008

From 2008.igem.org

Revision as of 11:30, 20 August 2008 by Andhi (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Edinburgh iGEM 2008

 

Week 8

Tuesday 5 August 08

  • M72 (pSB1A2+rbs+dxs), M79 (BABEL2+crtE) and M82 (pSB1A2+rbs+appY) submitted for seqencing. (CF)
  • Minipreps M97-104 of pSB1A2 cenA (2, 7-13) and M105-108 Minipreps M105-108 of pSB1A2+cex. (Yan)
  • M97-108 were double-digested with EcoRI/PstI and run on gel 37. (Yan)
  • Purification and self-ligation of P50 (3rd mutagenesis of glgc-mut1,2) (L30). (HX)
  • M68-69 (glgc-mut1,2 in pSB1A2) and M92 (pSB1A2+cex) were digested with a) PstI and b) EcoRI/PstI, but gel leaked. Entire process needs repeating tomorrow! (CF, OG)
  • Digested P36 (glgC-mut1,2) with Edinbrick1 using Spe1/EcoR1 - to be continued tomorrow. (OG)
  • Plate 93 created from subculture of plate 56 (M67, pSB1A2+rbs+crtB)
Retrieved from "http://2008.igem.org/Edinburgh/5_August_2008"