Team:Chiba/jk/β

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β班の実験ノート


Week 1

  • 17 August, 2008 : Transforation
  • BBa_R0040(2008) -> No colonies on plates
  • BBa_C0070(2008) -> No colonies on plates
  • BBa_C0076(2008) -> No colonies on plates
  • BBa_C0077(2008) -> No colonies on plates
  • BBa_C0078(2008) -> No colonies on plates
  • BBa_C0079(2008) -> No colonies on plates


  • 18 August, 2008 : Transformation
  • BBa_C0070(2007)
  • BBa_C0070(2006)
  • BBa_C0076(2007)
  • BBa_C0076(2006) -> No colonies on plates
  • BBa_C0078(2007)
  • BBa_C0078(2006)
  • BBa_C0170(2007)
  • BBa_C0170(2006)
  • BBa_C0178(2007)
  • BBa_C0178(2006)
  • 19 August, 2008
PCR Check
①BBa_I9026(2007)
②BBa_I9026(2006)
③BBa_I9030(2007)
④BBa_I9030(2006)
⑤BBa_S03154(2007)
⑥BBa_S03154(2006)
⑦BBa_F2620(2007)
  • 20 August, 2008
Mini prep SIGMA
  • BBa_C0070(2007)
  • BBa_C0070(2006)
  • BBa_C0076(2006)
  • BBa_C0078(2007)
  • BBa_C0078(2006)
Digestion Test
  • BBa_C0170(2007)
  • BBa_C0170(2006)
  • BBa_C0178(2007)
  • BBa_C0178(2006)
  • 21 August, 2008
PCR
  • BBa_C0070(2007)
  • BBa_C0070(2006)
  • BBa_C0076(2006)
  • BBa_C0078(2007)
  • BBa_C0078(2006)

Week 2

reference

  • L. Steindler.V.Venturi.:Detection of quorum sensing N-acyl homoserine lactone.FEMS Microbiol Lett 266 (2007) 1-9
クオラムセンシングのクロストークについてのミニレビュー。クオラムセンシングを利用したAHLバイオセンサーについて、Table 1.にまとまっている。
  • M.K Winson et al./FEMS Microbiology Letters 163 (1998) 185-192
Figure 2.hは、LuxR、RhlR、LasRそれぞれの、C4-C14のN-acyl-L-homoserine lactone,C4-C14のN-(3-oxoacyl)-L-homoserine lactoneに対する感受性を比較したグラフ。


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