Team:Chiba/jk/β

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β班の実験ノート


Contents

Week 1

  • 17 August, 2008
Transforation
  • [http://partsregistry.org/Part:BBa_R0040| BBa_R0040](2008) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2008) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2008) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0077| BBa_C0077](2008) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2008) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0079| BBa_C0079](2008) -> No colonies on plates


  • 18 August, 2008
Transforation
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007)
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006)
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007)
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2006) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007)
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006)
  • [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2007)
  • [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2006)
  • [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2007)
  • [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2006)


  • 19 August, 2008
PCR
  • [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2007)
  • [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2006)
  • [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2007)
  • [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006)
  • [http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2007)
  • [http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2006)
  • [http://partsregistry.org/Part:BBa_F2620| BBa_F2620](2007)･･･control
Gel Check
  • [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2007)
  • [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2006)
  • [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2007)
  • [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006)
  • [http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2007)
  • [http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2006)
  • [http://partsregistry.org/Part:BBa_F2620| BBa_F2620](2007)


  • 20 August, 2008
Mini prep
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007)
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006)
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007)
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007)
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006)
Digestion
  • [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2007)
  • [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2006)
  • [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2007)
  • [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2006)
Gel Check
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007)
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006)
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007)
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007)
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006)


  • 21 August, 2008
PCR
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007)
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006)
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007)
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007)
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006)
Gel Check
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007)
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006)
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007) -> None
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007) -> None
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006) -> None
Transforation
(Locate & 2 Punch ~iyama ver.~)
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2008) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0077| BBa_C0077](2008) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2008) -> No colonies on plates
Transforation
  • [http://partsregistry.org/Part:BBa_S03154| BBa_S03154](2007)
  • [http://partsregistry.org/Part:BBa_S03154| BBa_S03154](2006)
  • [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2007)
  • [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2006)
  • [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2007)
  • [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006)
  • 22 August, 2008


  • 23 August, 2008

Week 2

  • 24 August, 2008


  • 25 August, 2008


  • 26 August, 2008


  • 27 August, 2008
PCR
Colony PCR of 5 colonies from ligation plates (27/8) and one from control plate([http://partsregistry.org/Part:BBa_F2620| BBa_F2620](2007)).


  • 30 August, 2008


Week 3

  • 31 August, 2008


  • 1 September, 2008

Week 4

reference

  • L. Steindler.V.Venturi.:Detection of quorum sensing N-acyl homoserine lactone.FEMS Microbiol Lett 266 (2007) 1-9
クオラムセンシングのクロストークについてのミニレビュー。クオラムセンシングを利用したAHLバイオセンサーについて、Table 1.にまとまっている。
  • M.K Winson et al.Construction and analysis of luxCDABE-based plasmid sensors　for investigating N-acyl homoserine lactone-mediated quorum sensing.FEMS Microbiology Letters 163 (1998) 185-192
Figure 2.hは、LuxR、RhlR、LasRそれぞれの、C4-C14のN-acyl-L-homoserine lactone,C4-C14のN-(3-oxoacyl)-L-homoserine lactoneに対する感受性を比較したグラフ。
  • Michiko E. Taga. Bonnie L. Bassler.:Chemical communication among bacteria.PNAS.November 25, 2003,100suppl.2
クオラムセンシングについての総説。アシル基の異なるAHL、およびそのシンターゼの紹介、AIPを用いたコミュニケーション、AiiA（AHL分解酵素）についてのまとめがある。
  • K.M.GRAY et al.Interchangeability and Specificity of Components from the Quorum-Sensing Regulatory Systems of Vibrio fischeri and Pseudomonas aeruginosa.J.BACTERIOL.176,1994,3076-3080
LasとLuxのクロストークについて調べた論文。Table 1とTable 2で、promotor、レセプタータンパク質、インデューサーそれぞれを交換して、レポーターの発現の様子を調べている。
  • FK Balagadde et al.:A synthetic predator–prey ecosystem.Molecular Systems Biology 4:187,2008
LuxとLasによるクオラムセンシングを利用した、プレデター・プレイモデル。LuxとLasのクロストークについて言及あり。
  • Rong-guang Zhang et al.:Structure of a bacterial quorumsensing transcription factor complexed with pheromone and DNA.NATURE 417,2002,971-974
LuxR-familyタンパク質のTraR(Agrobacterium tumefaciens)の構造について、詳細な記述があります。
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