Revision as of 09:13, 24 September 2008 by Normanwang (Talk | contribs)

Birds Eye View Team	Projects	Events	Resources
Sponsors	Experiments	Milestones	Protocols
	Notebook (t)	Meetings (t)

Things we did today

Wetlab work

Grace

Margaret

1st lane is ladder, 2nd lane is rep amplified from a colony, 3rd lane is rep amplified from a restriction product of rep from the same colony.

Norman

x6 parallel MCS replacements fragment by PCR amplification. PCR out each MCS replacement insert with HindIII-VF2BB_fx._sb.1 and BamHI-VRBB_rx._sb.1
1. BBa_I52002 from pSB4A5 (Spring 2008 Plate 1022 1C)
2. BBa_I52001 from pSB4T5 (Spring 2008 Plate 1020 1A)
3. BBa_I52002 from BBa_I51020 (BBa_I51020 Base Vector from glycerol stock Inventory [http://packrat.stjohn.hawaii.edu/prestinglab/wiki/INV-Z80-R1-A3 INV-Z80-R1-A3])
4. BBa_P1010 from pSB1A3 (pSB1A3 from glycerol stock Inventory [http://packrat.stjohn.hawaii.edu/prestinglab/wiki/INV-Z80-R1-A3 INV-Z80-R1-A3])
5. BBa_P1010 from pSB1A7 (pSB1A7 from glycerol stock Inventory [http://packrat.stjohn.hawaii.edu/prestinglab/wiki/INV-Z80-R1-A3 INV-Z80-R1-A3])
6. BBa_J33207 from pSB1A2 ([http://partsregistry.org/wiki/index.php/Part:BBa_J33207 BBa_J33207] harboring pSB1A2 from plasmid prep)
x2 Controls
1. positive control: [http://partsregistry.org/wiki/index.php/Part:BBa_K125805 BBa_K125805] from pSB1A3 ([http://partsregistry.org/wiki/index.php/Part:BBa_K125805 BBa_K125805] harboring pSB1A3 from glycerol stock Inventory [http://packrat.stjohn.hawaii.edu/prestinglab/wiki/INV-Z80-R1-D4 INV-Z80-R1-D4])
2. negative control: H₂O

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]