pMPMT5, pMPMT5-AID, pMPMT5-AIDT7 and pMPMT5-AiD+T7 were transformed to E.coli Top10 carrying pZC. transformants were plated on 'TAXI' solid medium (LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal)