Transformation of E.coli Top10 carrying pZC320 with pMPMT5, pMPMT5-AID, pMPMT5-AIDT7 and pMPMT5-AiD+T7. Plating transformants on 'TAXI'(LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).