Team:Warsaw/Calendar-Main/20 June 2008

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Michał L., Ewa, Marcin

PCR
ProductTemplatePrimersProduct length
linker-ApDRIVE-TapTagAL+link10+homo2 and OmpaL_N 470 bp
alpha-linkerpUC19 OmpaL_N and AlphaP+link10+homo2600 bp
omega-linkerpUC19OmpaL_N and OmpaL_N400 bp

Universal PCR program for A, alpha and omega

TemperatureTime
94°C4:00
94°C0:3028 cycles
50°C0:45
72°C2:00
72°C10:00
4°Cinfinite

Polymerase: Pfu-Turbo

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