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Judging/Variance/Freiburg
From 2008.igem.org
Request
Dear iGEM-judging team,
representing 2008Žs team of the Albert-Ludwigs-Universität Freiburg, I am asking for permission to submit our parts in a non-standard vector, which is fully compatible to the standard cloning scheme (none of the Biobrick-restriction sites -except for pre- and suffix of the parts- occur in the vector), and which is in addition compatible with our extended restriction-site-system for fusion-proteins from last yearŽs proposal (details: http://parts.mit.edu/igem07/index.php/Freiburg07/report_fusion_parts ).
This is necessary, because we have to avoid the stop-codons generated by the Biobrick-scars. Unfortunately, the standard vector contains the additional restriction sites we use for our parts.
As said, the vector ('pMA' from Geneart used for iGEM orders) would feature full compatibility, and the parts we want to submit fulfill all criteria of the Biobrick-standard. If you give us permission and need further information about the vector (sequence?), please let me know.
Sincerely,
Philipp
Response
Dear Philipp,
Your approach sounds reasonable. Just to be clear, is the vector that you would be submitting your parts open source, and available for free distribution? If so, then please submit your vector as a new part (including documentation), so that the registry has a standard, freely available plasmid that is compatible with your system for fusion-proteins. With the new vector in the registry, it would be appropriate to use that vector to submit your parts.
Thanks you for writing and referencing the documentation, which was quite helpful.
iGEM judging team
