"
Team:Chiba/Calendar-Home/19 September 2008
From 2008.igem.org
10 September 2008 <|> 20 September 2008
Laboratory work
Team:Communication
-->(?/9) Gel Check
| Sample Name | J04500 | R0010 | C0161 | Cin |
| Sample DNA(μL) | 30 | 30 | 90 | ? |
| Loading Dye(μL) | 6 | 6 | 18 | ? |
| TOTAL(μL) | 36 | 36 | 108 | 24 |
--->Gel Extract
(各サンプル well３つずつ)
- note:ADB buffer添加後,37℃,6h
- --->Zymo Clean
- elution
- J0450-->27μL
- C0161-->11μL
--->Gel Check
- --->Ligation
| Sample Name | insert+vector | insert | vector |
| C0161(μL) | 3 | 3 | - |
| J04500(μL) | 4.5 | - | 4.5 |
| ligase(μL) | 1 | 1 | 1 |
| Buffer(invitrogen)(μL) | 3 | 2 | 2 |
| NFW?(μL) | 3.5 | 4 | 2.5 |
| TOTAL(μL) | 15 | 10 | 10 |
- --->Transformed above ligation onto a LB-amp plate.
- note
- competent cell:XL10GOLD
- DNA:1μL,SOC:120μL
--->(20/9)Mini prep
- time-delay test
- conditions
- strain
Senders:XL10GOLD
Receiver:JW1908
- temparature
30℃ and Room Temparature
Team:Output
colony PCR
ptet+LuxR(x16)
ptet+LasR(x7)
ptet+RhlR(x5)
ptet+cinR(x5)
