Team:Warsaw/Calendar-Main/11 June 2008

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Blue/white and rifampicin test

Michał L., Ewa, Piotr

  1. Transformation of E. coli TOP10 carrying pZC320 with:
  2. Plating transformants on 'TAXI' (LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).
  3. Inoculation of transformants to liquid LB + Tetracycline + Ampicillin 30 μg/mL +/- 0.1% Ara.


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