"
Team:Heidelberg/Notebook/Sensing Group/Notebook/4thweek
From 2008.igem.org
Revision as of 15:02, 26 October 2008 by Koljaschleich (Talk | contribs)
Contents |
Monday, 08/25/2008
- sequentiell and simultaneous digestion of pDk48 NcoI/NdeI and NcoI/KpnI
--> not successful
- preparation of O/N culture of pDK48 transformed cells
Tuesday, 08/26/2008
Cloning
- digestion of pDK48 with XbaI and PstI to check wether the vector is the right one
- digestion of Fusion-1 (from 08/21/2008) with NcoI/NdeI, with subsequent gelextraction
- Ligation of Fusion-1 and pDk48 and transformation into DH5a competent cells
LuxS Test
