Team:Heidelberg/Notebook/Sensing Group/Notebook/9thweek

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Contents

Tuesday, 09/30/2008

  • Miniprep of LuxS+pDK6 and F1/F2-Luxp-pBAD33
  • Digestion of GeneArt Fusion constructs with EcoRI/PstI and Gel-Extraction
  • Digestion of GeneArt Fusion constructs with EcoRI/PstI and Gel-Extraction

Wednesday, 10/01/2008

  • Miniprep of Fusion-LuxP-pBAD33 (both constructs)
  • Digestion of pSB1A3 with EcoRI,PstI,NdeI (3µl DNA, 40 µl total volume)
  • Digestion of pSB2K3 (10µl DNA, 20µl total volume) with EcoRI,PstI
PCR GeneArt Fusion constructs with Prefix/Suffix primer yielded no product. F1/F2 with prefix/speI primer were positive, but mutation strategy dropped later. A mix of LuxS and pDK6 was added on the gel to check if double transformation was successfull. Digestions of pSB1A3 and pSB2K3 with EcoRI/Pst were supposed to be used for subsequent standardization of parts

Thursday, 10/02/2008

Friday, 10/03/2008

Go to 10th week

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