Team:Hawaii/Notebook/2008-06-19
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Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Alkaline Lysis Miniprep
- Krystle & Margaret
- Plasmid prep was performed with Biobricks (E0040 (GFP) with amp100 from DH5alpha, J04430 (lac+GFP) with amp100 from DH5alpha, B0034 (RBS) with amp100 from GP's super competent cells, and B0024 (TT)with amp100 from DH5alpha) and plasmids (pUC18 with amp100 from DH5alpha, pBLUSCRIPT with amp100 from DH5alpha, and RP4 with Kan 50 from E. coli HB101).
Cryo-Stocks
- Krystle
- Made cryo-stocks of pBLUSCRIPT, pUC18, and B0024.
Drylab Work
Primer Discussion
- Krystle, Norman, & Margaret
- Discussed the format of primers
- worked on revising primers
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]