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Team:NTU-Singapore/Notebook/19 June 2008
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NTU@iGEM
Project
Introduction
Methodology
Team
Introduction
Team background
Notebook
Dry Lab
Introduction
ODEs used
Modeling Constructs
Parameters used
Deterministic Modeling
Sensitivity Analysis
Stochastic Modeling
Wetlab
Introduction
Materials and Equipment
Protocols
Verification of Detection & Lysis System
Our New Parts
Characterization
Introduction
Standard Promoters
Characterization of pLacI-GFP
pLacI-GFP images and clips
Characterization of pLsrA-YFP
New characterization Proposal
Parameter Estimation & Correlations
Acknowledgements
Thursday 19 June
Miniprep for SupD & T7ptag
Afternoon: these tasks are carried out for LacI promoter and supD:
Gel extraction
Ligation
Cloning
Inoculate overnight at 37 degrees C.