Team:Paris/Modeling/f3bis

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F3env.png
(see the considerations on the use of EnvZ)

We have [EnvZ]produced = {coefenv}expr(pTet) = {coefenv} ƒ1([aTc]i)

and [EnvZ]total = [EnvZ]b + [EnvZ]produced

and [FliA] = {coefFliA}expr(pBad) = {coefFliA} ƒ2([arab]i)

So, if we denote phosphorylated OmpR by OmpR*, we have

F3ompfromenv.jpg

that we can then introduce in the previous expression (see ƒ3) :

F3ompfinalenv.jpg



param signification unit value comments
[expr(pFlhDC)] expression rate of
pFlhDC with RBS E0032
nM.min-1 need for 20 mesures with well choosen values of [aTc]i
γGFP dilution-degradation rate
of GFP(mut3b)
min-1 0.0198
[GFP] GFP concentration at steady-state nM need for 20 mesures
(fluorescence) value of the observed fluorescence au need for 20 mesures
conversion conversion ratio between
fluorescence and concentration
nM.au-1 (1/79.429)



param signification
corresponding parameters in the equations
unit value comments
K21eff dissociation constant OmpR_-_EnvZ
K21eff
nM the literature [?] gives K21 =
n21 complexation order OmpR_-_EnvZ
n21
no dimension the literature [?] gives n21 =
{coefenv} coefficient due to the difference of the RBS and degradation rate between EnvZ and GFP
 ! not precised in the equations !
no dimension ! not precised in the equations ! watch out when writing the corresponding simulating program
[EnvZ]b "basal" presence of EnvZ
[EnvZ]b
nM the literature [?] gives, under high osmolarity, [EnvZ]b =
[OmpR]b "basal" presence of OmpR
[OmpR]b
nM the literature [?] gives, under high osmolarity, [OmpR]b =