Team:Freiburg/Parts

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All of our parts feature our expanded Pre- and Suffix for in frame cloning of fusion proteins [http://parts.mit.edu/igem07/index.php/Freiburg07/report_fusion_parts (more)]. A complete, detailed list of all parts submitted to the registry in this year´s project can be found [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Freiburg here], as well as the respective DNA-Sequences.

basic parts

  • Signal peptide

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157001 BBa_K157001]
Description:
This sequence mediates transportation of the protein to a translocational pore by an RNA–multiprotein complex, the signal recognition particle (SRP) when fused to the n-terminus of a fusion protein with a transmembrane region[1]. There, after a pause of translation, the signal sequence is released and translation and translocation of the nascent chain are restarted[2].
Source:
Human EGFR (ErbB-1) signal sequence; originally mediating membrane integration of the EGF-receptor (ErbB-1). Sequence taken from UniProtKB/Swiss-Prot entry P00533.
Gene synthesis by ATG:biosynthetics, optimized for expression in homo sapiens by iGEM-Team Freiburg 2008.
References:
[1] Walter P, Johnson AE: “Signal sequence recognition and protein targeting to the endoplasmic reticulum membrane.” Annu Rev Cell Biol 1994, 10:87-119
[2] Robert M Stroud, Peter Walter: “Signal sequence recognition and protein targeting”, Current Opinion in Structural Biology 1999, 9:754–759
AA sequence:
MRPSGTAGAA LLALLAALCP ASRA
gene sequence:
ATGAGACCAT CTGGTACTGC TGGAGCCGCA TTGCTGGCAC TTTTGGCTGC GCTGTGCCCT GCAAGCAGAG CA

  • Erbb1-transmembrane region

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157002 BBa_K157002]
Description:
Helical, single-span transmembrane region of the human EGF-Receptor type ErbB-1, sequence taken from UniProtKB/Swiss-Prot entry P00533.
source:
Transmembrane region of the human EGF-Receptor type ErbB-1. Sequence taken from UniProtKB/Swiss-Prot entry P00533. Gene synthesis by ATG:biosynthetics, optimized for expression in homo sapiens by iGEM-Team Freiburg 2008.
AA sequence:
ATGMVGALLL LLVVALGIGL FM
gene sequence:
ATAGCTACCG GAATGGTGGG TGCACTTTTG CTCCTTTTGG TCGTTGCCCT GGGGATAGGA CTCTTTATG

  • Sc-Fv anti NIP

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157003 BBa_K157003]
Description:
Singlechain Fv.Fragment of the Anti-Nitro-Iodo-Phenol-antibody B1-8. Binds the hapten NIP (Nitro-Iodo-Phenole) with an affinity of 2.0 uM[1,2]. Designed for fusion to proteins or peptides via in frame cloning.
source:
Gene synthesis by GeneArt, optimized for expression in homo sapiens.
References:
[1]Ana Cumano and Klaus Rajewski: “Clonal recruitment and somatic mutation in the generation of immunological memory to the hapten NP”, The EMBO Journal vol. 5 no.10 pp. 2459-2468, 1986
[2]D. Allen, T. Simon, F. Sablitzky, K. Rajewski and A. Cumano: “Antibody engineering for the analysis of affinity maturation of an anti-hapten response”, The EMBO Journal vol. 7 no.7 pp. 1995-2001, 1988
AASequence:
QVQLQQPGAE LVKPGASVKL SCKASGYTFT SYWMHWVKQR PGRGLEWIGR IDPNSGGTKY NEKFKSKATL TVDKPSSTAY MQLSSLTSED SAVYYCARYD YYGGSYFDYW GQGTTVTVSS GGGGSGGGGS GGGGSQAVVT QESALTTSPG ETVTLTCRSS TGAVTTSNYA NWVQEKPDHL FTGLIGGTNN RAPGVPARFS GSLIGDKAAL TITGAQTEDE AIYFCALWYS NHWVFGGGTK LTVLG
gene sequence:
CAGGTGCAGC TCCAGCAGCC CGGAGCCGAA CTGGTGAAGC CAGGCGCCAG CGTGAAGCTG TCCTGCAAGG CCAGCGGCTA CACCTTCACC AGCTACTGGA TGCACTGGGT GAAACAGAGG CCCGGCAGAG GCCTGGAATG GATCGGCCGG ATCGACCCCA ACAGCGGCGG CACCAAGTAC AACGAGAAGT TCAAGAGCAA GGCCACCCTG ACCGTGGACA AGCCCAGCAG CACCGCCTAC ATGCAGCTGT CCAGCCTGAC CAGCGAGGAC AGCGCCGTGT ACTACTGCGC CAGATACGAC TACTACGGCG GCAGCTACTT CGACTACTGG GGCCAGGGCA CCACCGTGAC CGTGTCCTCT GGGGGAGGGG GCTCAGGAGG AGGAGGAAGC GGGGGAGGGG GCAGCCAGGC CGTGGTGACC CAGGAAAGCG CCCTGACCAC TCCCCTGGCG GACAGTGACC TGACCTGCCG TCCTCTACAG GCGCCGTGAC CACAAGCAAC TACGCCAACT GGGTGCAGGA AAAGCCCGAC CACCTGTTCA CCGGCCTGAT CGGCGGCACA AACAACAGAG CCCCTGGCGT GCCCGCTAGA TTCAGCGGCA GCCTGATCGG GGACAAGGCC GCCCTGACAA TCACAGGCGC CCAGACCGAG GACGAGGCCA TCTACTTTTG CGCCCTGTGG TACAGCAACC ACTGGGTGTT CGGCGGAGGG ACCAAGCTGA CCGTGCTGGG C

  • LipocalinFluA

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157004 BBa_K157004]
Description:
Fluoresceine A -binding derivative of a Lipocalin (BBP, bilin binding protein from pieris brassicae), originally designed by Arne Skerra[1,2].
Source:
Gene synthesis by GeneArt, optimized for expression in homo sapiens.
References:
[1] Gerald Beste, Frank S. Schmidt, Thomas Stibora and A. Skerra: “Small antibody-like proteins with prescribed ligand specificities derived from the lipocalin fold“,Proc. Natl. Acad. Sci. USA Vol. 96, pp. 1898–1903, March 1999 Biochemistry
[2]Ingo P. Korndörfer, Gerald Beste and A. Skerra: “Crystallographic Analysis of an “Anticalin” With Tailored Specificity for Fluorescein Reveals High Structural Plasticity of the Lipocalin Loop Region”, PROTEINS: Structure, Function, and Bioinformatics 53:121–129 (2003)
AA sequence:
MAGDVYHDGA CPEVKPVDNF DWSQYHGKWW EVAKYPSPNG KYGKCGWAEY TPEGKSVKVS RYDVIHGKEY FMEGTAYPVG DSKIGKIYHS RTVGGYTRKT VFNVLSTDNK NYIIGYSCRY DEDKKGHWDH VWVLSRSMVL TGEAKTAVEN YLIGSPVVDS QKLVYSDFSE AACKVNNTG
gene sequence:
GACGTGTACC ACGACGGCGC CTGCCCCGAA GTGAAGCCCG TGGACAACTT CGACTGGTCC CAGTACCACG GCAAGTGGTG GGAGGTGGCC AAGTACCCCA GCCCCAACGG CAAGTATGGC AAGTGCGGCT GGGCCGAGTA CACCCCCGAG GGCAAGAGCG TGAAGGTGTC CAGATACGAC GTGATCCACG GCAAAGAATA CTTCATGGAA GGCACCGCCT ACCCCGTGGG CGACAGCAAG ATCGGCAAGA TCTACCACAG CCGGACCGTG GGCGGCTACA CCAGAAAGAC CGTGTTCAAC GTGCTGTCCA CCGACAACAA GAACTACATC ATCGGCTACA GCTGCCGCTA CGACGAGGAC AAGAAGGGCC ACTGGGACCA CGTGTGGGTG CTGTCCCGGT CCATGGTGCT GACCGGCGAG GCCAAGACCG CCGTGGAGAA CTACCTGATC GGCAGCCCCG TGGTGGACAG CCAGAAACTG GTGTACAGCG ACTTCAGCGA GGCCGCCTGC AAAGTGAACA AC

  • Split-Cerulean-C-CFP

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157005 BBa_K157005]
Description:
C-terminal fragment (Amino acids156-236) of the monomeric[5] cyan fluorescent protein variant “Cerulean”, designed for fusion/BiFC[1,3]. Reassembly with Split-Cerulean-nCFP (Part Bba_K157006) generates the complete, working CFP “Cerulean”, whereas combination with the N-terminal fragment (Part Bba_K157008) of the monomeric yellow fluorescent Protein “Venus”[4] (Part Bba_I757008) results in green fluorescence[2].
AASequence:
KNGIKANFKI RHNIEDGSVQ LADHYQQNTP IGDGPVLLPD NHYLSTQSKL SKDPNEKRDH
gene sequence:
MVLLEFVTAA GITLGMDELY KAAGAACGGC ATCAAGGCCA ACTTCAAGAT CCGGCACAAC ATCGAGGATG GCAGCGTGCA GCTGGCCGAT CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGAGCAAGCT GTCCAAGGAC CCCAACGAGA AGCGGGACCA CATGGTGCTG CTGGAATTTG TGACAGCCGC CGGAATCACC CTGGGCATGG ACGAGCTGTA CAAG

  • Split-Cerulean-N-CFP

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157006 BBa_K157006]
Description:
N-terminal fragment (Amino acids1-155) of the monomeric[5] cyan fluorescent protein variant “Cerulean”, designed for fusion/BiFC[1,3]. Reassembly with Split-Cerulean-cCFP (Part Bba_K157005) generates the complete, working CFP “Cerulean”, whereas combination with the C-terminal fragment (Part Bba_K157007) of the monomeric yellow fluorescent Protein “Venus”[4] (Part Bba_I757008) results in green fluorescence[2].
AASequence:
MSKGEELFTG VVPILVELDG DVNGHKFSVS GEGEGDATYG KLTLKFICTT GKLPVPWPTL VTTLGVQCFA RYPDHMKRHD FFKSAMPEGY VQERTIFFKD DGNYKTRAEV KFEGDTLVNR IELKGIDFKE DGNILGHKLE YNAISDNVYI TADKQ
gene sequence:
ATGAGCAAGG GCGAGGAACT GTTCACCGGC GTGGTGCCCA TCCTGGTGGA GCTGGACGGC GACGTGAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AAGGCGACGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTG GTGACCACCC TGGGCGTGCA GTGCTTCGCC AGATACCCCG ACCACATGAA GCGGCACGAT TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAGGAAC GGACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG GCGACACACT GGTGAACCGG ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAATA TCCTGGGCCA CAAGCTGGAA TACAACGCCA TCAGCGACAA CGTGTACATC ACCGCCGACA GCAG

  • Split-Venus-C-YFP

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157007 BBa_K157007]
Description:
C-terminal fragment (Amino acids157-237) of the monomeric[5] yellow fluorescent protein variant “Venus” [4], designed for fusion/BiFC[1,3]. Reassembly with Part Bba_K157008 (Split-Venus-nYFP) generates the complete, working YFP “Venus”, whereas combination with the n-terminal fragment (Part Bba_K157006) of the monomeric cyan fluorescent Protein “CeruleanR206K” (Part Bba_I757009) results in green fluorescence[2].
AASequence:
KNGIKANFKI RHNIEDGGVQ LADHYQQNTP IGDGPVLLPD NHYLSYQSKL SKDPNEKRDH MVLLEFVTAA GITHGMDELY K
gene sequence:
AAGAACGGCA TCAAGGCCAA CTTCAAGATC CGGCACAACA TCGAGGACGG CGGCGTGCAG CTGGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCTACCA GAGCAAGCTG TCCAAGGACC CCAACGAGAA GCGGGACCAC ATGGTGCTGC TGGAATTTGT GACAGCCGCC GGAATCACCC ACGGCATGGA CGAGCTGTAC AAG

  • Split-Venus-N-YFP

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157008 BBa_K157008]
Description:
N-terminal fragment (Amino acids1-156) of the monomeric[5] yellow fluorescent protein variant “Venus” [4], designed for fusion/BiFC[1,3]. Reassembly with Part Bba_K157007 (Split-Venus-cYFP) generates the complete, working YFP “Venus”, whereas combination with the C-terminal fragment (Part Bba_K157005) of the monomeric cyan fluorescent Protein “CeruleanR206K” (Part Bba_I757009) results in green fluorescence[2].
AASequence:
MVSKGEELFT GVVPILVELD GDVNGHKFSV SGEGEGDATY GKLTLKLICT TGKLPVPWPT LVTTLYLQCF ARYPDHMKQH DFFKSAMPEG YVQERTIFFK DDGNYKTRAE VKFEGDTLVN RIELKGIDFK EDGNILGHKL EYNYNSHNVY ITADKQ
gene sequence:
ATGGTGTCCA AGGGCGAGGA ACTGTTCACC GGCGTGGTGC CCATCCTGGT GGAGCTGGAC GGCGACGTGA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAAGGCGA CGCCACCTAC GGCAAGCTGA CCCTGAAGCT GATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTGGTGACCA CCCTGTACCT CCAGTGCTTC GCCAGATACC CCGACCACAT GAAGCAGCAC GATTTCTTCA AGAGCGCCAT GCCCGAGGGC TACGTGCAGG AACGGACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC CAGAGCCGAA GTGAAGTTCG AGGGCGACAC ACTGGTGAAC CGGATCGAGC TGAAGGGCAT CGACTTCAAA GAGGACGGCA ATATCCTGGG CCACAAGCTG GAATACAACT ACAACAGCCA CAACGTGTAC ATCACCGCCG ACAAGCAG

Source (all split-fluorophores):
Gene synthesis by GeneArt, optimized for expression in homo sapiens.
References (all split-fluorophores):
- [1] Chang-Deng Hu, Yurii Chinenov, Tom K. Kerppola: ”Visualization of Interactions among bZIP and Rel Family Proteins in Living Cells Using Bimolecular Fluorescence Complementation”, Molecular Cell, Vol. 9, 789–798, April, 2002
- [2] Chang Deng Hu, Tom K. Kerppola: “Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis”, Nat Biotechnol. 2003 May; 21(5):539-545 (doi:10. 1038/nbt816)
-[3] Tom K. Kerppola: “Design and implementation of bimolecular fluorescence complementation (BiFC) assays for the visualization of protein interactions in living cells”, Nat Protoc. 2006;1(3):1278-1286 (doi:10.1038/nprot.2006.201)
-[4]Nagai, T. et al. “A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications” J. Biol. Chem. 276, 29188-29194, 2001
-[5]Roger Y. Tsien et al. „Creating new fluorescent probes for cell biology“, Nature Biotechnology Reviews, Vol. 3, 906-918, 2002

  • Split-Fluo-Linker

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157009 BBa_K157009]
description:
Originally, this linker was used for fusion to the N-terminus of the C-terminal half of split fluorophores: Protein interactions can be examined by BiFC[1] using complementary, non-fluorescent fragments of fluorophores[2]. Therefore, it is essential that the C-terminal fragment of the fluorophore is not restricted too much in its mobility. The linker allows orientation and adaption of the C-terminal fragment to the N-terminal fragment of the split fluorophore and, thus, the reassembly of a working fluorescent protein. It has already been used in BiFC assays and is known to serve this purpose well[3]; anyway, another, more flexible linker that could be used instead is our “GGGGS-linker” (Part Bba_K157010).
References:
- [1] Chang-Deng Hu, Yurii Chinenov, Tom K. Kerppola: ”Visualization of Interactions among bZIP and Rel Family Proteins in Living Cells Using Bimolecular Fluorescence Complementation”, Molecular Cell, Vol. 9, 789–798, April, 2002
- [2] Chang Deng Hu, Tom K. Kerppola: “Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis”, Nat Biotechnol. 2003 May; 21(5):539-545 (doi:10. 1038/nbt816)
-[3] Tom K. Kerppola: “Design and implementation of bimolecular fluorescence complementation (BiFC) assays for the visualization of protein interactions in living cells”, Nat Protoc. 2006;1(3):1278-1286 (doi:10.1038/nprot.2006.201)
source:
Gene synthesis by ATG:biosynthetics, optimized for expression in homo sapiens by iGEM-Team Freiburg 2008.
AA Sequence:
RPACKIPNDL KQKVMNH
gene sequence:
CGACCAGCCT GTAAGATTCC AAATGACCTG AAGCAGAAAG TTATGAATCA C

  • GGGGS-LINKER_BCR-Transmem.

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157010 BBa_K157010]
Description:
Helical single-span transmembrane region of the B-Cell-Receptor with a flexible 15 aa Linker at the N-terminus. Designed for fusion to proteins or peptides that are to be presented at the cells surface; signal peptide for membrane integration (e.g. part Bba_K157001) required at the N-terminus of the whole construct!

  • His-Tag (improved)

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157011 BBa_K157011]
See Part BBa_I757013 (Freiburg 2007)

  • Strep-Tag II (improved)

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157012 BBa_K157012]
See Part BBa_I757014 (Freiburg 2007)

  • GGGS-linker (pMA)

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157013 BBa_K157013]
Description:
A flexible, 15 aa long linker designed for fusion to/of proteins or peptides via in frame cloning. Consists of Glycine and Serine only.
source:
Gene synthesis by ATG:biosynthetics, optimized for expression in homo sapiens by iGEM-Team Freiburg 2008.
AA sequence:
GGGSGGGSGG GSGGG
gene sequence:
GGTGGAGGAG GTTCTGGAGG CGGTGGAAGT GGTGGCGGAG GTAGC


composite parts

  • Tm-Fluo-linker-cYFP

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157014 BBa_K157014]
Description:
C-terminal part of the Split fluorophore YFP fused to the trans-membrane region of the Erbb1/EGF-receptor with the fluolinker in between. Part was derived by cloning of BBa_K157002, BBa_K157009 and BBa_K157007. For further information see the description of the parts mentioned above.
AA sequence:
MAGIATGMVG ALLLLLVVAL GIGLFMTGRP ACKIPNDLKQ KVMNHTGKNG IKANFKIRHN IEDGGVQLAD HYQQNTPIGD GPVLLPDNHY LSYQSKLSKD PNEKRDHMVL LEFVTAAGIT HGMDELYKTG*
gene sequence:
ATGGCCGGCA TAGCTACCGG AATGGTGGGT GCACTTTTGC TCCTTTTGGT CGTTGCCCTG GGGATAGGAC TCTTTATGAC CGGCCGACCA GCCTGTAAGA TTCCAAATGA CCTGAAGCAG AAAGTTATGA ATCACACCGG CAAGAACGGC ATCAAGGCCA ACTTCAAGAT CCGGCACAAC ATCGAGGACG GCGGCGTGCA GCTGGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCTACC AGAGCAAGCT GTCCAAGGAC CCCAACGAGA AGCGGGACCA CATGGTGCTG CTGGAATTTG TGACAGCCGC CGGAATCACC CACGGCATGG ACGAGCTGTA CAAGACCGGT TAA

  • Tm-nYFP

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157015 BBa_K157015]
Description:
N-terminal part of the Split-flourophore YFP, fused to the trans-membrane region of the Erbb1/EGF-receptor. Part was derived by cloning of BBa_K157002, BBa_K157008. For further information see the description of the parts mentioned above.
AA sequence:
MAGIATGMVG ALLLLLVVAL GIGLFMTGMV SKGEELFTGV VPILVELDGD VNGHKFSVSG EGEGDATYGK LTLKLICTTG KLPVPWPTLV TTLYLQCFAR YPDHMKQHDF FKSAMPEGYV QERTIFFKDD GNYKTRAEVK FEGDTLVNRI ELKGIDFKED GNILGHKLEY NYNSHNVYIT ADKQTG*
gene sequence:
ATGGCCGGCA TAGCTACCG GAATGGTGGG TGCACTTTTG CTCCTTTTGG TCGTTGCCCT GGGGATAGGA CTCTTTATGA CCGGCATGGT GTCCAAGGGC GAGGAACTGT TCACCGGCGT GGTGCCCATC CTGGTGGAGC TGGACGGCGA CGTGAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAA GGCGACGCCA CCTACGGCAA GCTGACCCTG AAGCTGATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTGGT GACCACCCTG TACCTCCAGT GCTTCGCCAG ATACCCCGAC CACATGAAGC AGCACGATTT CTTCAAGAGC GCCATGCCCG AGGGCTACGT GCAGGAACGG ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCAGAG CCGAAGTGAA GTTCGAGGGC GACACACTGG TGAACCGGAT CGAGCTGAAG GGCATCGACT TCAAAGAGGA CGGCAATATC CTGGGCCACA AGCTGGAATA CAACTACAAC AGCCACAACG TGTACATCAC CGCCGACAAG CAGACCGGTT AA

  • Tm-Fluolinker-cCFP

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157016 BBa_K157016]
Description:
C-terminal part of Split-CFP, fused to the trans-membrane region of the Erbb1/EGF-receptor with the fluolinker in between. Part was derived by cloning of BBa_K157002, BBa_K157009 and BBa_K157005. For further information see the description of the parts mentioned above.
AA sequence:
MAGIATGMVG ALLLLLVVAL GIGLFMTGRP ACKIPNDLKQ KVMNHTGKNG IKANFKIRHN IEDGSVQLAD HYQQNTPIGD GPVLLPDNHY LSTQSKLSKD PNEKRDHMVL LEFVTAAGIT LGMDELYKTG*
gene sequence:
ATGGCCGGCA TAGCTACCGG AATGGTGGGT GCACTTTTGC TCCTTTTGGT CGTTGCCCTG GGGATAGGAC TCTTTATGAC CGGCCGACCA GCCTGTAAGA TTCCAAATGA CCTGAAGCAG AAAGTTATGA ATCACACCGG CAAGAACGGC ATCAAGGCCA ACTTCAAGAT CCGGCACAAC ATCGAGGATG GCAGCGTGCA GCTGGCCGAT CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGAGCAAGCT GTCCAAGGAC CCCAACGAGA AGCGGGACCA CATGGTGCTG CTGGAATTTG TGACAGCCGC CGGAATCACC CTGGGCATGG ACGAGCTGTA CAAGACCGGT TAA

  • Tm-nCFP

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157017 BBa_K157017]
Description:
N-terminal part of Split-CFP, fused to the trans-membrane region of the Erbb1/EGF-receptor. Part was derived by cloning of BBa_K157002, BBa_K157006. For further information see the description of the parts mentioned above.
AA sequence:
XXX
gene sequence:
XXX

  • Tm-betaLactamaseBla1

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157018 BBa_K157018]
Description:
First fragment of beta-Lactamase fused to the trans-membrane region of the Erbb1/EGF-receptor. Part was derived by cloning of the parts BBa_K157002, BBa_I757011. For further information see the description of the parts mentioned above.
AA sequence:
MAGIATGMVGALLLLLVVALGIGLFMTGHPETLAKVKDAEDQLGARVGYIELDLNSGKIL ESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGM TVGELCSAAITMSDNTAANLLLTTIGGPKELTAFLRNMGDHVTRLDRWEPELNEAIPNDE RDTTTPVAMATTLRKLLTGELLGTG*
gene sequence:
´ATGGCCGGCATAGCTACCGGAATGGTGGGTGCACTTTTGCTCCTTTTGGTCGTTGCCCTG GGGATAGGACTCTTTATGACCGGCCACCCGGAAACCCTGGCCAAAGTGAAAGATGCGGAA GATCAGCTGGGTGCGCGTGTGGGCTATATTGAACTGGATCTGAACAGCGGCAAAATTCTG GAATCTTTTCGTCCGGAAGAACGTTTTCCGATGATGAGCACCTTTAAAGTGCTGCTGTGC GGTGCGGTTCTGAGCCGTATTGATGCGGGCCAGGAACAGCTGGGCCGTCGTATTCATTAT AGCCAGAACGATCTGGTGGAATATAGCCCGGTGACCGAAAAACATCTGACCGATGGCATG ACCGTGGGCGAACTGTGCAGCGCGGCGATTACCATGAGCGATAACACCGCGGCGAACCTG CTGCTGACCACCATTGGCGGTCCGAAAGAACTGACCGCGTTTCTGCGTAACATGGGCGAT CATGTGACCCGTCTGGATCGTTGGGAACCGGAACTGAACGAAGCGATTCCGAACGATGAA CGTGATACCACCACCCCGGTGGCCATGGCGACCACCCTGCGTAAACTGCTGACCGGCGAA CTGCTGGGCACCGGTTAA

  • Tm-betaLactamase(Bla2)

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157019 BBa_K157019]
Description:
Second fragment of beta-Lactamase fused to the trans-membrane region of the Erbb1/EGF-receptor. Part was derived by cloning of BBa_K157002, BBa_I757012. For further information see the description of the parts mentioned above.
AA sequence:
XXX
gene sequence:
XXX

  • Tm-Split-Luciferase1/2

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157020 BBa_K157020]
Description:
Part 1 of the Split- Luciferase(BB057) fused to the trans-membrane region of the Erbb1/EGF-receptor. Part was derived by cloning of BBa_K157002 and Splitluciferase kindly supported by Raik Gruenberg. For further information see the description of the parts mentioned above.
AA sequence:
XXX
gene sequence:
XXX

  • Tm-Splitluciferase2/2

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157021 BBa_K157021]
Description:
Second part of Split-Luciferase(BB058) fused to the trans-membrane region of the Erbb1/EGF-receptor. Part was derived by cloning of the parts BBa_K157002 and Splitluciferase kindly supported by Raik Gruenberg. For further information see the description of the parts mentioned above.
AA sequence:
XXX
gene sequence:
XXX

  • SP-AntiNIP-GGGSlinker

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157022 BBa_K157022]
Description:
The scFv-anti-NIP (with a flexible GGGS-linker at the C-terminus) fused to the signal peptide of Erbb1/EGF-receptor. Part was derived by cloning of BBa_K157001, BBa_K157003 and BBa_K157013. For further information see the description of the parts mentioned above.
AA sequence:
XXX
gene sequence:
XXX

  • SP-Lipocalin-GGGSlinker

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157023 BBa_K157023]
Description:
Lipocalin (with a flexible GGGS-linker at the C-terminus) fusioned to the signal peptide of Erbb1/EGF-receptor. Part was derived by cloning of BBa_K157001, BBa_K157003 and BBa_K157013. For further information see the description of the parts mentioned above.
AA sequence:
XXX
gene sequence:
XXX

  • SP-AntiNIP-GGGSlinker-Tm-betaLactamase(Bla1)

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157024 BBa_K157024]
Description:
A composite part consisting of the signal-peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of split-enzyme beta-Lactamase. Part was derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002 and BBa_I757011. For further information see the description of the parts mentioned above.
AA sequence:
XXX
gene sequence:
XXX

  • SP-AntiNIP-GGGSlinker-Tm-betaLactamase(Bla2)

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157025 BBa_K157025]
Description:
A composite part consisting of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split-enzyme beta-Lactamase. Part was derived by cloning of BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002 and BBa_I757012. For further information see the description of the parts mentioned above.
AA sequence:
XXX
gene sequence:
XXX

  • SP-AntiNIP-GGGSlinker-Tm-Fluolinker-cYFP

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157026 BBa_K157026]
Description:
A composite part consisting of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus. Part was derived by cloning of BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157009 and BBa_K157007. For further information see the description of the parts mentioned above.
AA sequence:
XXX
gene sequence:
XXX

  • SP-AntiNIP-GGGSlinker-Tm-nYFP

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157027 BBa_K157027]
Description:
Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of the split-fluorophor YFP. Part was derived by cloning of BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157008. For further information see the description of the parts mentioned above.
AA sequence:
XXX
gene sequence:
XXX

  • SP-AntiNIP-GGGSlinker-Tm-Fluolinker-cCFP

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157028 BBa_K157028]
Description:
Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus. Part was derived by cloning of BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157009 and BBa_K157005. For further information see the description of the parts mentioned above.
AA sequence:
XXX
gene sequence:
XXX

  • SP-AntiNIP-GGGSlinker-Tm-nCFP

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157029 BBa_K157029]
Description:
Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and intracellular the N-terminal part of the split fluorophor YFP. Part was derived by cloning of BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157006. For further information see the description of the parts mentioned above.
AA sequence:
XXX
gene sequence:
XXX

  • SP-AntiNIP-GGGSlinker-Tm-Splitluciferase2/2

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157030 BBa_K157030]
Description:
Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and one half of the Splitluciferase(BB058). Part was derived by cloning of BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002 and Splitluciferase kindly supported by Raik Gruenberg. For further information see the description of the parts mentioned above.
AA sequence:
XXX
gene sequence:
XXX

  • SP-AntiNIP-GGGSlinker-Tm-Splitluciferase1/2

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157031 BBa_K157031]
Description:
Part consists of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and one half of the Splitluciferase(BB058). Part was derived by cloning of BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002 and Splitluciferase kindly supported by Raik Gruenberg. For further information see the description of the parts mentioned above.
AA sequence:
XXX
gene sequence:
XXX

  • SP-Lipocalin-GGGSlinker-Tm-betaLactamase(Bla1)

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157032 BBa_K157032]
Description:
Part consists of the signal peptide of Erbb1/EGF-receptor, Lipocalin, GGGSflexlinker , the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of the split enzyme beta-Lactamase. Part was derived by cloning of BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002 and BBa_I757011. For further information see the description of the parts mentioned above.
AA sequence:
XXX
gene sequence:
XXX

  • SP-Lipocalin-GGGSlinker-Tm-betaLactamase(Bla2)

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157033 BBa_K157033]
Description:
Part consists of the signal peptide of Erbb1/EGF-receptor, Lipocalin, GGGSflexlinker, the transmembrane region of the Erbb1/EGF-receptor and the C-terminal part of the split enzyme beta-Lactamase. Part was derived by cloning of BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002 and BBa_I757012. For further information see the description of the parts mentioned above.
AA sequence:
XXX
gene sequence:
XXX

  • SP-Lipocalin-GGGSlinker-Tm-Fluolinker-cYFP

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157034 BBa_K157034]
Description:
A composite part consisting of the signal peptide of Erbb1/EGF-receptor, Lipocalin, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus. Part was derived by cloning of BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002, BBa_K157009 and BBa_K157007. For further information see the description of the parts mentioned above.
AA sequence:
XXX
gene sequence:
XXX

  • SP-Lipocalin-GGGSlinker-Tm-nYFP

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157035 BBa_K157035]
Description:
A composite part consisting of the signal peptide of Erbb1/EGF-receptor, Lipocalin, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of the split fluorophor YFP. Part was derived by cloning of the parts BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002, BBa_K157008. For further information see the description of the parts mentioned above.
AA sequence:
XXX
gene sequence:
XXX

  • SP-Lipocalin-GGGSlinker-Tm-Fluolinker-cCFP

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157036 BBa_K157036]
Description:
A composite part consisting of the signal peptide of Erbb1/EGF-receptor, Lipocalin, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor CFP with a fluolinker joined to its N-Terminus. Part was derived by cloning of BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002, BBa_K157009 and BBa_K157005. For further information see the description of the parts mentioned above.
AA sequence:
XXX
gene sequence:
XXX

  • SP-Lipocalin-GGGSlinker-Tm-nCFP

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157037 BBa_K157037]
Description:
A composite part consisting of the signal peptide of Erbb1/EGF-receptor, Lipocalin, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of the split fluorophor CFP. Part was derived by cloning of BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002, BBa_K157006. For further information see the description of the parts mentioned above.
AA sequence:
XXX
gene sequence:
XXX


  • SP-Lipocalin-GGGSlinker-Tm-Splitluciferase2/2

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157038 BBa_K157038]
Description:
A composite part consisting of the signal peptide of Erbb1/EGF-receptor, Lipocalin, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and one half of the Splitluciferase. Part was derived by cloning of BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002 and Splitluciferase kindly supported by Raik Gruenberg. For further information see the description of the parts mentioned above.
AA sequence:
XXX
gene sequence:
XXX

  • SP-Lipocalin-GGGSlinker-Tm-Splitluciferase1/2

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157039 BBa_K157039]
Description:
A composite part consisting of the signal peptide of Erbb1/EGF-receptor, Lipocalin, GGGSflexlinker, the trans-membrane region of the Erbb1/EGF-receptor and one half of Splitluciferase. Part was derived by cloning of BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002 and Splitluciferase kindly supported by Raik Gruenberg. For further information see the description of the parts mentioned above.
AA sequence:
XXX
gene sequence:
XXX

  • Transfection_Vector-(BBa_J52017)-CMV

Part Name:
[http://partsregistry.org/Part:BBa_K157040 BBa_K157040]
Description:
Transfection vector with CMV Promotor for expression in eukaryotic cells.
AA sequence:
XXX
gene sequence:
XXX

  • Transfection_Vector-(BBa_J52017)-CMV-CFP

Part Name:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K157041 BBa_K157041]
Description:
Transfectionvector with CMV promoter and CFP as reporter.
AA sequence:
XXX
gene sequence:
XXX

Freiburg08 FT3.png

Retrieved from "http://2008.igem.org/Team:Freiburg/Parts"