User contributions
From 2008.igem.org
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- 15:09, 17 July 2008 (diff | hist) Virginia/17 July 2008 (→Goals)
- 14:59, 17 July 2008 (diff | hist) Virginia/17 July 2008 (→Goals)
- 14:50, 17 July 2008 (diff | hist) Virginia/17 July 2008 (→Goals)
- 14:27, 17 July 2008 (diff | hist) N Virginia/17 July 2008 (New page: ==Overnight Results== *only plate with R0010 +A4 grew which is the same result as from the night before **Which leads us to think our assemblies have some problem *the assembly of BP2 and ...)
- 14:57, 15 July 2008 (diff | hist) N Virginia/15 July 2008 (New page: ==Goals== *GET GFP EXPRESSION! *miniprep DNA **Screening plasmid (psb1A10) **Assembly 3 (I715039 and E0240) **BP 2 **BP 3 *Assemble parts **Repressible Promoter(R0040) and GFP(E0240) **Sta...) (top)
- 14:46, 15 July 2008 (diff | hist) Virginia/14 July 2008 (top)
- 19:34, 14 July 2008 (diff | hist) N Virginia/14 July 2008 (New page: ==Goals== *turn in time sheets! *Examine flourescent proteins under flourescence camera **psb1A10 **assembly 3 of i715039 and E0240 *make overnight broths of these flourescent protein cell...)
- 19:23, 14 July 2008 (diff | hist) Virginia/11 July 2008 (top)
- 14:40, 8 July 2008 (diff | hist) N Virginia/8 July 2008 (New page: ==Goals== *Prepare overnight broth of successful single colony of Promoter + RBS transformation *Plate Screening plasmid (psb1a10) when it arrives from iGem *Try again with ligation of RFP...)
- 14:26, 8 July 2008 (diff | hist) N Virginia/7 July 2008 (New page: ==Goals== *Buy plane tickets *Reserve hotel room *Promoter + RBS ligation *Promoter + RBS transformation and plating ==Results== *have been having problems with purification of small part...) (top)
- 14:52, 1 July 2008 (diff | hist) N Virginia/1 July 2008 (New page: ==Goals== *digest parts *do not purify parts *heat deactivate digestion enzyme *ligate *transform Notebook) (top)
- 14:49, 1 July 2008 (diff | hist) N Virginia/27 June 2008 (New page: ==Goals== *small broth of plated parts *minipreps of parts that are going to be assembled *redigest all the assembly parts *run gels to verify purified cut outs *if possible run 3A assembl...) (top)
- 14:39, 30 June 2008 (diff | hist) N Virginia/28 June 2008 (New page: *Take out incubated over night brothes *spin down cultures to pellets to store in freezer Notebook) (top)
- 14:37, 30 June 2008 (diff | hist) N Virginia/30 June 2008 (New page: ==Goals== *Order midiprep kit *miniprep ligation of BP1 and terminator (Assembly 2) *Digest ligation *run regular gel of ligation and large purified parts that will be used in other ligati...)
- 15:35, 27 June 2008 (diff | hist) m Virginia/26 June 2008 (top)
- 15:29, 27 June 2008 (diff | hist) Virginia/26 June 2008
- 15:26, 27 June 2008 (diff | hist) m Virginia/26 June 2008 (→Notes)
- 15:25, 27 June 2008 (diff | hist) N Virginia/26 June 2008 (New page: ==Goals== *small broth of plated terminator *large broth of E0240(RFP) and I715039(GFP) *mini prepe PRE RFP and RET GFP for another ligation *redigest 6 parts for the three ligations *pu...)
- 15:13, 27 June 2008 (diff | hist) Virginia/25 June 2008 (top)
- 14:33, 25 June 2008 (diff | hist) Virginia/25 June 2008
- 14:32, 25 June 2008 (diff | hist) Virginia/25 June 2008
- 14:32, 25 June 2008 (diff | hist) Virginia/25 June 2008
- 14:31, 25 June 2008 (diff | hist) Virginia/25 June 2008
- 14:28, 25 June 2008 (diff | hist) N Virginia/5 July 2008 (New page: 50% Off Fireworks!!! Notebook) (top)
- 14:27, 25 June 2008 (diff | hist) N Virginia/6 July 2008 (New page: Everything Must Go Fireworks!!! Notebook) (top)
- 14:26, 25 June 2008 (diff | hist) N Virginia/4 July 2008 (New page: BIG Fireworks!!! Notebook) (top)
- 14:26, 25 June 2008 (diff | hist) N Virginia/6 June 2008 (New page: BIG Fireworks!!! Notebook)
- 14:25, 25 June 2008 (diff | hist) N Virginia/3 July 2008 (New page: Fireworks!!! Notebook) (top)
- 14:25, 25 June 2008 (diff | hist) N Virginia/2 July 2008 (New page: Fireworks!!! Notebook) (top)
- 14:22, 25 June 2008 (diff | hist) Virginia/24 June 2008 (top)
- 14:20, 25 June 2008 (diff | hist) Virginia/23 June 2008 (top)
- 14:17, 25 June 2008 (diff | hist) Virginia/24 June 2008
- 14:15, 25 June 2008 (diff | hist) Virginia/25 June 2008
- 14:15, 25 June 2008 (diff | hist) N Virginia/25 June 2008 (New page: Goals: --make small broth of plated part -B1002 -E0840 stil does not grow --Digest, Purify and ligate RFP(I715039) and GFP(E0240) for a 3a assembly with the standard back bone psb1a3 wit...)
- 17:33, 24 June 2008 (diff | hist) Virginia/24 June 2008
- 17:32, 24 June 2008 (diff | hist) N Virginia/24 June 2008 (New page: Goals: --Small overnight broths of E0240 and I17039 --Finish 3A ass. by ligating digested parts --pour more Amp agar plates --Transform more cells (BBa_B1002) --Order more supplies-pu...)
- 14:15, 23 June 2008 (diff | hist) Virginia/23 June 2008
- 14:09, 23 June 2008 (diff | hist) Virginia/23 June 2008
- 14:08, 23 June 2008 (diff | hist) N Virginia/23 June 2008 (New page: Goals: -Retrieve lost Igem newsletter -design test plasmid with program -Inquire about bad parts (BBa_E0240 and psB1A10) -complete 3A assembly with first BP gene)
- 18:34, 19 June 2008 (diff | hist) N Virginia/20 June 2008 (New page: *Goto Jordan Hall and get more db3.1 cells *learn how to run protein gel *Test microscope with the 2 plates *3A assembly *Order 2nd and 3rd BP genes [[Team:Virginia/Notebook|Notebo...) (top)
- 18:29, 19 June 2008 (diff | hist) Virginia/19 June 2008 (top)
- 14:37, 18 June 2008 (diff | hist) N Virginia/18 June 2008 (New page: Goals: -Maxiprep more genes -Retransform failed plates from day before -Pellet large broths -Email Igem to send repeated failed genes -Finish presentation -Read article on BP measur...) (top)
- 14:25, 18 June 2008 (diff | hist) N Virginia/14 June 2008 (New page: had to have someone come in to take out over night cultures and dishes from incubation Team:Virginia/Notebook) (top)
- 14:23, 18 June 2008 (diff | hist) Virginia/17 June 2008 (top)
- 14:23, 18 June 2008 (diff | hist) Virginia/16 June 2008 (top)
- 14:23, 18 June 2008 (diff | hist) Virginia/28 May 2008 (top)
- 14:22, 18 June 2008 (diff | hist) Virginia/19 May 2008 (top)
- 14:21, 18 June 2008 (diff | hist) N Virginia/28 May 2008 (New page: Adviser presentation 1)
- 14:18, 18 June 2008 (diff | hist) Virginia/19 May 2008
- 14:18, 18 June 2008 (diff | hist) N Virginia/19 May 2008 (New page: First day in the lab introduction to materials in the lab and a few protocols that will be necessary -making LB broth -Making and pouring agar plates -where supplies were stored (drawers,...)
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