User contributions
From 2008.igem.org
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- 07:28, 13 August 2008 (diff | hist) N Team:NTU-Singapore/Notebook/12 August 2008 (New page: =Tuesday 12 August= *Lu Chao: inoculate 6xLysis-Standard Vector and 6xLsrA-Standard Vector)
- 08:21, 24 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook/24 July 2008
- 08:06, 24 July 2008 (diff | hist) N Team:NTU-Singapore/Notebook/24 July 2008 (New page: =Thursday 24 July= *RESULT from Wednesday ligation and transformation: **E7-Term got 7 colonies!! **Lysis-standard vector and LsrA-standard vector got lots of colonies!! **Lysis-Term also...)
- 08:01, 24 July 2008 (diff | hist) N Team:NTU-Singapore/Notebook/23 July 2008 (New page: =Wednesday 23 July= Hung, Min: ==Digestion for Terminator with X/P on 2% gel== *As we could not see the band of Terminator insert on Tuesday to extract) *Gel loading and extraction + nanod...)
- 07:32, 24 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook/22 July 2008
- 07:31, 24 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook/22 July 2008
- 06:19, 24 July 2008 (diff | hist) Nm Team:NTU-Singapore/Notebook/22 July 2008 (New page: =Tuesday 22 July= Hung,Lu Chao, Min: ==Ligation of E7 (vector) with Terminator (insert)== *E7: cut with S/P for 2 hours *Terminator: cut with X/P for 2 hours)
- 06:16, 24 July 2008 (diff | hist) N Team:NTU-Singapore/Notebook/21 July 2008 (New page: =Monday 21 July= Hung, Lu Chao: ==Ligation of Lysis (insert) and Terminator (vector):== *For Terminator (V): cut with E/X in Buffer2 for 1 hour, and 2 hours (digestion time check) *For Lys...)
- 06:00, 24 July 2008 (diff | hist) N Team:NTU-Singapore/Notebook/19 July 2008 (New page: =Saturday 19 July= Darius: *Minipreps for E7 and T7ptag inoculated on Friday. *Run PCR for checking E7. *Inoculation for Lysis and LsrA cells.)
- 05:57, 24 July 2008 (diff | hist) N Team:NTU-Singapore/Notebook/18 July 2008 (New page: =Friday 18 July= *RESULTS FROM THURSDAY TRANSFORMATION: **Plate of E7 got 4 colonies!! **Plate of T7ptag got 8 colonies!! *We also received LsrA and Lysis (lyophilized form) in Bluescript ...)
- 05:52, 24 July 2008 (diff | hist) N Team:NTU-Singapore/Notebook/17 July 2008 (New page: =Thursday 17 July= *Ligate E7 and T7ptag(cut with E/P, gel extracted on Wednesday) into Empty vector. *MinElute PCR purification. *Transform into competent cells. (use all 10ul of each lig...)
- 05:48, 24 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook/16 July 2008
- 05:11, 24 July 2008 (diff | hist) N Team:NTU-Singapore/Notebook/16 July 2008 (New page: =Wednesday 16 July= *Morning: We carried out several digestion: **GFP with E/P in buffer 2 for 1 hour; in buffer 2 for 2 hours; and in EcoRI buffer for 2 hours (this aims to compare the ef...)
- 11:34, 15 July 2008 (diff | hist) N Team:NTU-Singapore/Notebook/15 July 2008 (New page: *All 4 plates of Fe-GFP from Monday had colonies. We did the inoculation in the afternoon. *Ligation of T7ptag, E7 with empty vector from GFP: **We already got E7 (cut with E/P), E7 (cut w...)
- 11:18, 15 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook/14 July 2008
- 11:12, 15 July 2008 (diff | hist) Nm Team:NTU-Singapore/Notebook/13 July 2008 (New page: *Darius: inoculation for E7-AmpR vector and T7ptag-AmpR vector.)
- 11:10, 15 July 2008 (diff | hist) Team:NTU-Singapore/Notebook/11 July 2008
- 15:47, 10 July 2008 (diff | hist) Nm Team:NTU-Singapore/Notebook/10 July 2008 (New page: =Thursday 10 July= *1015-1215: Hung, Lu Chao: minipreps and nanodrops for LacI-GFP 1ul (x3), LacI-GFP 4ul (x2), LacI-RBS 1:3, LacI-RBS 1:6, Fe-GFP 1ul(x3), Fe-GFP 4ul (x1). The microliter ...)
- 19:21, 9 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook/9 July 2008
- 19:20, 9 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook/9 July 2008
- 15:02, 9 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook/9 July 2008
- 14:08, 8 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook/8 July 2008
- 14:08, 8 July 2008 (diff | hist) Team:NTU-Singapore/Notebook/8 July 2008
- 16:24, 7 July 2008 (diff | hist) N Team:NTU-Singapore/Notebook/7 July 2008 (New page: =Monday 7 July= *Results from last week: **1 tube of LacI-RBS 34 was obtained! **Cells with LacI-RFP plasmids in fact appeared in pink color. And the color looks basically the same as the ...)
- 18:22, 5 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook/5 July 2008
- 18:19, 5 July 2008 (diff | hist) N Team:NTU-Singapore/Notebook/5 July 2008 (New page: =Saturday 5 July= *Among the plates collected, only the plate of LacI-RFP has colonies. Plates with E7-empty plasmid and LacI-GFP do not have colony. *Lu Chao: minipreps for LacI-RBS 34. *...)
- 18:10, 5 July 2008 (diff | hist) N Team:NTU-Singapore/Notebook/4 July 2008 (New page: =Friday 4 July= *Hung, Darius: **Ligation of E7 into empty plasmid vector extracted from GFP plasmid. ***Gel electrophoresis for the E7 plasmids synthesized by PCR (with 2 blunt ends recog...)
- 17:56, 5 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook/3 July 2008
- 17:42, 4 July 2008 (diff | hist) m Team:NTU-Singapore
- 15:03, 4 July 2008 (diff | hist) N File:NTU igem team members.JPG (top)
- 02:32, 3 July 2008 (diff | hist) Team:NTU-Singapore (→Graduate Student Advisors)
- 02:12, 3 July 2008 (diff | hist) Team:NTU-Singapore (→Team background)
- 11:32, 2 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook/2 July 2008
- 11:30, 2 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook/1 July 2008 (→Tuesday 1 July)
- 17:03, 1 July 2008 (diff | hist) m Team:NTU-Singapore
- 14:48, 1 July 2008 (diff | hist) m Team:NTU-Singapore/Acknowledgements
- 09:34, 1 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook/30 June 2008
- 09:29, 1 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook/1 July 2008
- 09:29, 1 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook/1 July 2008
- 09:16, 1 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook/1 July 2008
- 09:08, 1 July 2008 (diff | hist) N Team:NTU-Singapore/Notebook/1 July 2008 (New page: =Tuesday 1 July= *Hung, Darius, Choon Kit: try out ligation of pFe(insert) with GFP(vector); and pLacI(insert) with RBS34(vector): **1030-1130:prepare digestion mixtures for pFe,GFP,pLacI,...)
- 08:34, 1 July 2008 (diff | hist) m Team:NTU-Singapore/Notebook
- 15:19, 30 June 2008 (diff | hist) m Team:NTU-Singapore (→Project introduction)
- 16:51, 28 June 2008 (diff | hist) m Team:NTU-Singapore
- 16:50, 28 June 2008 (diff | hist) m Team:NTU-Singapore
- 16:48, 28 June 2008 (diff | hist) N File:NTU logo ver3.jpg (top)
- 16:43, 28 June 2008 (diff | hist) m Team:NTU-Singapore
- 16:32, 28 June 2008 (diff | hist) N File:NTU logo ver3.gif (NTU logo version 3) (top)
- 05:12, 28 June 2008 (diff | hist) N Team:NTU-Singapore/Notebook/28 June 2008 (New page: =Saturday 28 June= *Hung: 10:30am to 12:30 pm: miniprep for 6 samples of pFe-GFP)
- 14:10, 27 June 2008 (diff | hist) N Team:NTU-Singapore/Notebook/27 June 2008 (New page: =Saturday 27 June= *Hung: miniprep for pFe,Terminator,RBS32,RBS34,pT7,GFP,empty plasmid AmpR,LacI *Choon Kit: inoculate pFe-GFP)
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