Chin Chong

• Stock check of items purchased so far and shifted them into new lab with Darius's help
• Received quotation for LsrA-RBS and Lysis sequencing from Science Werke, confirmed the purchase and sequence should arrive in 2-4 weeks
• Purchased items from SBS general store
• Did inoculation of colonies from Top cells containing:

1. LacI-GFP [http://partsregistry.org/wiki/index.php?title=Part:BBa_I763004 BBa_I763004],
2. Standard promoter with GFP reporter [http://partsregistry.org/Part:BBa_I20260 BBa_I20260]
3. GFP reporter devices with Weak [http://partsregistry.org/Part:BBa_J23150 BBa_J23150],
4. Medium [http://partsregistry.org/Part:BBa_J23151 BBa_J23151] and
5. Strong [http://partsregistry.org/Part:BBa_J23102 BBa_J23102] promoters

• Incubate cells in 5ml of LB broth containing the respective anitbiotics and allow them to grow overnight
  • This is the slight modification from the standard protocol suggested in the Newsletter Vol.1 which used supplemented M9 medium instead of LB broth
  • M9 medium with glycerol will be introduced in the following day after the cells have been allowed to grow overnight in LB

Choon Kit, Zhen Fu,Hung:

• Nanodrop to measure concentrations and purities of pFe,pLacI,GFP,RBS32,RBS34,pT7,Terminator. The data show a ratio of A260/A280 varying around 1.85 to 1.95, which mean the purities are quite good.