User contributions
From 2008.igem.org
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- 17:39, 28 October 2008 (diff | hist) File:6-28 mxh 4.jpg (uploaded a new version of "Image:6-28 mxh 4.jpg") (top)
- 17:39, 28 October 2008 (diff | hist) File:6-28 mxh 3.jpg (uploaded a new version of "Image:6-28 mxh 3.jpg") (top)
- 17:39, 28 October 2008 (diff | hist) File:6-28 mxh 2.jpg (uploaded a new version of "Image:6-28 mxh 2.jpg") (top)
- 17:39, 28 October 2008 (diff | hist) File:6-28 mxh 1.jpg (uploaded a new version of "Image:6-28 mxh 1.jpg") (top)
- 17:39, 28 October 2008 (diff | hist) File:6-27 redigest TA.jpg (uploaded a new version of "Image:6-27 redigest TA.jpg") (top)
- 17:38, 28 October 2008 (diff | hist) File:6-25 digest.jpg (uploaded a new version of "Image:6-25 digest.jpg") (top)
- 17:34, 28 October 2008 (diff | hist) Team:Harvard/Dailybook/Week0 (top)
- 17:33, 28 October 2008 (diff | hist) Team:Harvard/Dailybook/Week0
- 17:32, 28 October 2008 (diff | hist) Team:Harvard/Dailybook/Week0
- 23:19, 26 October 2008 (diff | hist) Team:Harvard/Notebook
- 23:19, 26 October 2008 (diff | hist) Team:Harvard/Notebook
- 19:54, 26 October 2008 (diff | hist) N File:16s PCR Gel2(MR1 + Ecoli).jpg
- 19:54, 26 October 2008 (diff | hist) N File:16s PCR Gel1(S1).jpg
- 19:54, 26 October 2008 (diff | hist) N File:6-28 mxh 5.jpg
- 19:54, 26 October 2008 (diff | hist) N File:6-28 mxh 4.jpg
- 19:53, 26 October 2008 (diff | hist) N File:6-28 mxh 3.jpg
- 19:53, 26 October 2008 (diff | hist) N File:6-28 mxh 2.jpg
- 19:53, 26 October 2008 (diff | hist) N File:6-28 mxh 1.jpg
- 19:53, 26 October 2008 (diff | hist) N File:6-27 redigest TA.jpg
- 19:53, 26 October 2008 (diff | hist) N File:6-25 digest.jpg
- 19:49, 26 October 2008 (diff | hist) Team:Harvard/Dailybook/Week0 (→Transformed S1 via Electroporation)
- 19:48, 26 October 2008 (diff | hist) Team:Harvard/Dailybook/Week0 (→Transformed S1 via Electroporation)
- 19:48, 26 October 2008 (diff | hist) Team:Harvard/Dailybook/Week0 (→Transformed S1 via Electroporation)
- 19:47, 26 October 2008 (diff | hist) Team:Harvard/Dailybook/Week0 (→Protocols)
- 19:47, 26 October 2008 (diff | hist) Team:Harvard/Dailybook/Week0 (→Tuesday: June 17, 2008)
- 19:47, 26 October 2008 (diff | hist) Team:Harvard/Dailybook/Week0 (→Tuesday: June 17, 2008)
- 19:45, 26 October 2008 (diff | hist) N File:PACYC-map.pdf (top)
- 19:44, 26 October 2008 (diff | hist) N Team:Harvard/Plasmids (New page: == Vectors == *pACYC-Duet vector map *[http://partsregistry.org/wiki/index.php?title=Part:pSB3K3 pSB3K3] ==Parts== *[http://spreadsheets.google.com/ccc?key=pwvx...)
- 19:44, 26 October 2008 (diff | hist) Team:Harvard/Notebook (→Notebooks)
- 19:42, 26 October 2008 (diff | hist) Team:Harvard/Team
- 19:41, 26 October 2008 (diff | hist) Team:Harvard/Team (→Advisors)
- 19:40, 26 October 2008 (diff | hist) Team:Harvard/Team (→Teaching Fellows)
- 19:40, 26 October 2008 (diff | hist) Team:Harvard/Team
- 19:39, 26 October 2008 (diff | hist) Team:Harvard/Team
- 19:35, 26 October 2008 (diff | hist) Team:Harvard/Notebook (→Notebooks)
- 19:35, 26 October 2008 (diff | hist) N Team:Harvard/Recipes (New page: =Recipes= ==Orianna's growth media== * 50 mM K-PIPES '''18.925 g/L''' * 7.5 mM NaOH '''7.5 mL/L''' * 28 mM NH<sub>4</sub>Cl '''1.5 g/L''' * 1.3 mM KCl ...)
- 19:34, 26 October 2008 (diff | hist) N Team:Harvard/PrelimTest (New page: =Week 0= ==OD and GFP expression in ''E. Coli''== June 17, 2008 ===3 hrs in Incubation=== {| {{table}} | align="center" style="background:#eeeeee;"|'''Cell Type''' | align="center" style=...) (top)
- 19:32, 26 October 2008 (diff | hist) N Team:Harvard/MFCProtocols (New page: =Running an MFC Experiment from Start to Finish= This page is intended as a comprehensive guide to completing a microbial fuel cell experiment ==Creating a Testing Environment== Begin ...) (top)
- 19:32, 26 October 2008 (diff | hist) Team:Harvard/Notebook
- 19:31, 26 October 2008 (diff | hist) N MFCProtocols (New page: =Running an MFC Experiment from Start to Finish= This page is intended as a comprehensive guide to completing a microbial fuel cell experiment ==Creating a Testing Environment== Begin ...) (top)
- 19:26, 26 October 2008 (diff | hist) N Team:Harvard/GenProtocols (New page: ==QIAprep Spin Miniprep Kit== This protocol is designed for the purification of up to 20 ug high-copy plasmid DNA from 1-5 ml overnight ''E. coli'' culture in LB medium. ===Procedure=== #...)
- 19:26, 26 October 2008 (diff | hist) Team:Harvard/Notebook
- 19:24, 26 October 2008 (diff | hist) N GenProtocols (New page: ==QIAprep Spin Miniprep Kit== This protocol is designed for the purification of up to 20 ug high-copy plasmid DNA from 1-5 ml overnight ''E. coli'' culture in LB medium. ===Procedure=== #...) (top)
- 19:22, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week14/Chemical and Light (New page: =Mystery Colony PCR 10/08= Colony PCR of putative fungal colonies using primers BBpfx and mtrB500. Image:10-08_Mystery_PCR_MXHTA.jpg Lane 1: 1 kb ladder Lanes 2-6: P136 plate (500 b...) (top)
- 19:22, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week13/Chemical and Light (New page: =Colony PCR 09/30= Should grow only on carb *1-30: 20, 23, 25 (picked all three) --> P38+130 *31-60: 31-60 (picked 31, 35, 39, 42, 46, 50, 55, 60) --> P39+130 Should grow only on kan *61-...) (top)
- 19:22, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week12/Chemical and Light (New page: =9/22 RE digests= With NEB enzymes, 60 min (buffer 2+BSA): *128#1 XPXmnI *128#5 XSXmnI *128#9 ESXmnI The XmnI is to cut the backbone, which has a similar length to the mtrB constructs. W...)
- 19:21, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week11/Chemical and Light (New page: <b>Summary of the week</b> * Retransformed plasmids containing low expression ompR and HO-pcyA (for light system) w/ Cm resistance * Ligated mtrB after an RBS and before a terminator (2 se...) (top)
- 19:21, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week10/Chemical and Light (New page: =Transformations in Shewie= About 250 ng of DNA added each time. ==First Set 8/22== {| {{table}} | align="center" style="background:#f0f0f0;"|'''Plate''' | align="center" style="backgrou...) (top)
- 19:20, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week9/Chemical and Light (New page: =105, mtrB Ligations/transformations 08/17= Parts to ligated came from 8/17 We used a 2:1 molar ratio of insert to vector for all of the ligations. The ligations were all transformed into...) (top)
- 19:20, 26 October 2008 (diff | hist) N Team:Harvard/Dailybook/Week8/Chemical and Light (New page: =MIT lacZ parts RE digests 08/11= {| {{table}} !rowspan=8|Image:8-11_gel_1_MXHTA.jpg !colspan=2 align="center" style="background:#f0f0f0;"|'''1% agarose''' |- | align="center" style="b...) (top)
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