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User contributions
From 2008.igem.org
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- 18:14, 28 October 2008 (diff | hist) N Ligate DNA (New page: ==Ligation Protocol==)
- 18:12, 28 October 2008 (diff | hist) Team:Princeton/Experiments (→Bio-brick Creation)
- 18:11, 28 October 2008 (diff | hist) N CIP Treatment (New page: ==CIP Protocol==)
- 18:10, 28 October 2008 (diff | hist) N PCR SOEing (New page: ==PCR SOEing Protocol==)
- 18:09, 28 October 2008 (diff | hist) N Primer Design (New page: ==Primer Design Protocol==) (top)
- 18:08, 28 October 2008 (diff | hist) N Restriction Map/ Restriction Digest (New page: ==Restriction Map Protocol== ==Restriction Digest Protocol==)
- 18:05, 28 October 2008 (diff | hist) Run Gel/ Gel extraction
- 18:01, 28 October 2008 (diff | hist) PCR Amplification
- 18:00, 28 October 2008 (diff | hist) PCR Amplification
- 17:51, 28 October 2008 (diff | hist) N Sequence (New page: ==Sequencing Protocol== '''Protocol:''' Go to Genewiz.com to order sequencing. Sequencing primers should be diluted to 5uM; 4.0ul of 5uM primer should be used. Depending on plasmid size...)
- 17:39, 28 October 2008 (diff | hist) Extract DNA (Maxiprep or Midiprep) (→Midiprep Protocol (QIAGEN Miniprep kit))
- 17:38, 28 October 2008 (diff | hist) Extract DNA (Maxiprep or Midiprep)
- 17:37, 28 October 2008 (diff | hist) N Extract DNA (Maxiprep or Midiprep) (New page: ==Midiprep Protocol (QIAGEN Miniprep kit)== 1. Transfer the overnight culture of plasmid cells into 2ml microcentrifuge collection tubes (1 per try). Pellet for 3 min at >8000 rpm in a c...)
- 17:35, 28 October 2008 (diff | hist) N Re-transform with selected plasmid (New page: ==Transformation Protocol== Before you start, make sure the water bath is at 42 degrees and the SOC is at 37 degrees. If the SOC is cloudy or has anything floating in it, it has gone bad ...)
- 17:34, 28 October 2008 (diff | hist) Team:Princeton/Experiments (→Bio-brick Creation)
- 17:33, 28 October 2008 (diff | hist) N Extract DNA (Miniprep) (New page: ==Miniprep Protocol (QIAGEN Miniprep kit)== All centrifugation steps are done at 13000 rpm, after step 2, in a table-top microcentrifuge 1. Transfer the overnight culture of plasmid cel...)
- 17:32, 28 October 2008 (diff | hist) N Transformation and Plating (New page: ==Transformation Protocol== Before you start, make sure the water bath is at 42 degrees and the SOC is at 37 degrees. If the SOC is cloudy or has anything floating in it, it has gone bad...)
- 17:28, 28 October 2008 (diff | hist) N PCR Purification (New page: ==PCR Purification== Using QIAquick kit 1. Add 5 volumes of Buffer PBI to 1 volume of sample. 2. Pipette into a QIAquick spin column(max 770 µl) and centrifuge for 60 sec at 10,000g ...)
- 17:27, 28 October 2008 (diff | hist) N Digestion (New page: ==Digestion Protocol== 1. Use X units of enzymes per 1ug of DNA (over digesting by factor of X) Usually we use over digest factor of 10 unless otherwise specified in the enzyme tech ...)
- 17:25, 28 October 2008 (diff | hist) Run Gel/ Gel extraction
- 17:24, 28 October 2008 (diff | hist) Run Gel/ Gel extraction
- 17:20, 28 October 2008 (diff | hist) Run Gel/ Gel extraction
- 17:19, 28 October 2008 (diff | hist) N Run Gel/ Gel extraction (New page: Gel Extraction Protocol using QIAquick Gel Extraction Kit: All centrifugation steps are done at speeds > 13000 rpm 1. Look at the gel under low wavelength UV (high wavelengths will denat...)
- 17:17, 28 October 2008 (diff | hist) Team:Princeton/Experiments (→Bio-brick Creation)
- 17:12, 28 October 2008 (diff | hist) PCR Amplification (→PCR Protocol)
- 17:11, 28 October 2008 (diff | hist) N PCR Amplification (New page: ==PCR Protocol== '''Material:''' Primers (FWD, RVS) PCR Supermix Parent plasmid '''Method:''' 1. Measure the concentration of the primers and the parent plasmid as follow: Do a dilutio...)
- 17:10, 28 October 2008 (diff | hist) Team:Princeton/Experiments (→Bio-Brick Verification)
- 17:09, 28 October 2008 (diff | hist) Team:Princeton/Experiments (→Bio-brick Creation)
- 17:06, 28 October 2008 (diff | hist) Team:Princeton/Experiments (→Bio-brick Creation)
- 17:01, 28 October 2008 (diff | hist) Team:Princeton/Experiments (→Bio-brick Creation)
- 08:51, 28 October 2008 (diff | hist) Team:Princeton/ProjectDetails (→Experiments)
- 08:49, 28 October 2008 (diff | hist) Team:Princeton/Experiments (→Bio-brick Creation)
- 08:43, 28 October 2008 (diff | hist) Team:Princeton/Experiments (→Bio-brick Creation)
- 08:41, 28 October 2008 (diff | hist) Team:Princeton/Experiments (→Bio-Brick Verification)
- 08:27, 28 October 2008 (diff | hist) Team:Princeton/Experiments (→Bio-Brick Verification)
- 08:27, 28 October 2008 (diff | hist) Team:Princeton/Experiments (→Bio-brick Creation)
- 08:26, 28 October 2008 (diff | hist) Team:Princeton/Experiments (→Bio-brick Creation)
- 08:25, 28 October 2008 (diff | hist) Team:Princeton/Experiments (→Bio-brick Creation)
- 08:25, 28 October 2008 (diff | hist) Team:Princeton/Experiments (→Bio-brick Creation)
- 08:23, 28 October 2008 (diff | hist) Team:Princeton/Experiments (→Bio-brick Creation)
- 08:19, 28 October 2008 (diff | hist) Team:Princeton/Experiments
- 12:44, 15 August 2008 (diff | hist) Team:Princeton/Talia Dan-Cohen
- 11:22, 15 August 2008 (diff | hist) Team:Princeton/Lena Phalen (top)
- 20:54, 14 August 2008 (diff | hist) Team:Princeton/Katia Sherman
- 20:53, 14 August 2008 (diff | hist) Team:Princeton/Katia Sherman
- 20:52, 14 August 2008 (diff | hist) Team:Princeton/Katia Sherman
- 12:51, 1 August 2008 (diff | hist) Caroline Richardson (top)
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