User contributions
From 2008.igem.org
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- 20:15, 28 October 2008 (diff | hist) N File:SNCLavalin.PNG (top)
- 20:14, 28 October 2008 (diff | hist) N File:EngFac.PNG (top)
- 20:13, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 20:11, 28 October 2008 (diff | hist) N File:ChemEng.PNG (top)
- 20:10, 28 October 2008 (diff | hist) N File:NEB.PNG (top)
- 20:09, 28 October 2008 (diff | hist) N File:FSF.PNG (top)
- 20:08, 28 October 2008 (diff | hist) N File:DeptBio.PNG (top)
- 20:08, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 20:04, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 20:03, 28 October 2008 (diff | hist) N File:SciFac.PNG (top)
- 20:03, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 19:58, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 19:46, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 19:43, 28 October 2008 (diff | hist) N File:MEF.PNG (top)
- 19:18, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 19:08, 28 October 2008 (diff | hist) Team:Waterloo/Team
- 19:07, 28 October 2008 (diff | hist) m Team:Waterloo/Team
- 18:57, 28 October 2008 (diff | hist) Team:Waterloo/Team
- 18:30, 28 October 2008 (diff | hist) Team:Waterloo/Team
- 18:07, 28 October 2008 (diff | hist) N File:Adam Stinchcombe.PNG (top)
- 18:06, 28 October 2008 (diff | hist) Team:Waterloo/Team
- 18:03, 28 October 2008 (diff | hist) Team:Waterloo/Team
- 18:00, 28 October 2008 (diff | hist) N File:Vanja Polic.PNG (top)
- 18:00, 28 October 2008 (diff | hist) Team:Waterloo/Team
- 17:53, 28 October 2008 (diff | hist) File:Emily Wedland.PNG (uploaded a new version of "Image:Emily Wedland.PNG": Reverted to version as of 17:18, 28 October 2008) (top)
- 17:49, 28 October 2008 (diff | hist) File:Emily Wedland.PNG (uploaded a new version of "Image:Emily Wedland.PNG")
- 17:48, 28 October 2008 (diff | hist) Team:Waterloo/Team
- 17:18, 28 October 2008 (diff | hist) N File:Emily Wedland.PNG
- 17:17, 28 October 2008 (diff | hist) Team:Waterloo/Team
- 14:53, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 14:51, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 14:47, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 14:08, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 14:05, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 13:53, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 13:31, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 13:09, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 19:35, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Making SOB Media (New page: <big><b><u>Making SOB Medium</u></b></big><br> Used in growing bacteria for preparing chemically competent cells <br> <b>Ingredients </b><br> - 0.5% (w/v) yeast extract <br> - 2% (w/v)...)
- 19:28, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Protocols/Competent Cells (New page: <big><b><u>Recipe for Competent Cells </u></b></big> <br> <b>Materials </b><br> Frozen stock DH5a <br> 250mL Erlenmeyer Flask (w/ caps) <br> LB tubes <br> Sterile LB <br> <b>Instructio...)
- 19:08, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Making LB Media (New page: <big><b><u>Making LB Media</u></b></big> <br> <b>Materials </b><br> 500 mL media bottle or rack of test tubes <br> 1 L beaker <br> Magnetic stir bar <br> Tryptone <br> Yeast extract<br> ...)
- 18:59, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Making AI Media (New page: <b><u><big>Making Auto Induction (AI) Media</big></u></b><br> <b>Before you start </b><br> Certain parts are on variable copy number plasmids. The plasmid is normally low copy number, b...)
- 18:47, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Protocols/Transformation (New page: <b><u><big>Transformation</big></u></b><br> <b>Before you start </b><br> Cells lose their competence once thawed, so be sure to:<br> a) keep them on ice at all times.<br> b) use cells imm...)
- 18:26, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Protocols/Inoculation (New page: <big><b><u>Inoculation Protocol </u></b></big><br> <b>Materials </b><br> DNA Samples <br> Ampicillin (2µL/sample)<br> Kanamycin (2.5µL/sample)<br> Liquid LB Media (1 tube/sample)<br> <...)
- 18:20, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Protocols/Glycerol Stock Preparation (New page: <b><u><big>Glycerol Stock Preparation </big></u></b><br> <b>Before you start </b><br> - It is important to use aseptic technique when carrying out the procedures <br> <b>Materials </b><...)
- 18:10, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Protocols/Ligation (New page: <big><b><u>Ligation (10µL recipe with 5 insert : 1 vector ratio)</u></b></big> <br> <b>Materials </b><br> MQ water <br> Ligase buffer<br> Ligase <br> Eppendorf tube<br> Vector DNA<br> I...)
- 18:03, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Protocols/Gel Electrophoresis (New page: <b><u><big>Gel Electrophoresis</big></u></b><br> <b>Materials </b><br> Agarose <br> 1X TAE buffer <br> Gel Red<br> 1:10 diluted 1kb DNA ladder<br> Loading Dye<br> Gel Rig<br> <b>Instru...)
- 17:43, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Protocols/DNA Extraction (New page: <b><u><big>DNA Extraction </big></u></b><br> <b>Materials </b><br> All required materials provided in the Fermentas DNA Extraction Kit (K0513)<br> <b>Instructions </b><br> 1. Follow the ...)
- 17:31, 27 October 2008 (diff | hist) Team:Waterloo/Notebook (→Protocols)
- 17:31, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Protocols/DNA Digestion (New page: <b><u><big>Digestion of Plasmid DNA </big></u></b><br> <b>Before you start </b><br> - Know what is the total digestion volume mix. From that you can determine the enzyme concentration whi...)
- 17:25, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Diagnostic Digestion (New page: <b><u><big>Diagnostic Digestion of Plasmid DNA </big></u></b><br> <b>Before you start </b><br> - Know what is the total digestion volume mix. From that you can determine the enzyme concen...) (top)
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