User contributions
From 2008.igem.org
(Latest | Earliest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)
- 02:07, 29 October 2008 (diff | hist) Wisconsin: Lignin Project/1 July 2008 (top)
- 02:02, 29 October 2008 (diff | hist) Wisconsin: Lignin Project/30 June 2008 (top)
- 02:02, 28 October 2008 (diff | hist) Andy's Profile (top)
- 01:52, 28 October 2008 (diff | hist) Mattias's Profile (top)
- 01:45, 28 October 2008 (diff | hist) Andy's Profile
- 23:20, 8 September 2008 (diff | hist) Andy's Profile
- 10:37, 25 August 2008 (diff | hist) Team:Wisconsin/Project
- 10:30, 25 August 2008 (diff | hist) Team:Wisconsin/Project
- 10:28, 25 August 2008 (diff | hist) Andy's Profile
- 20:32, 5 August 2008 (diff | hist) Team:Wisconsin/Team
- 20:32, 5 August 2008 (diff | hist) Team:Wisconsin/Team
- 23:26, 31 July 2008 (diff | hist) Team:Wisconsin/Team
- 23:20, 31 July 2008 (diff | hist) Team:Wisconsin
- 23:21, 14 July 2008 (diff | hist) Wisconsin: Lignin Project/25 June 2008
- 23:10, 14 July 2008 (diff | hist) Wisconsin: Lignin Project/24 June 2008
- 23:02, 14 July 2008 (diff | hist) Wisconsin: Lignin Project/23 June 2008
- 23:01, 14 July 2008 (diff | hist) Wisconsin: Lignin Project/23 June 2008
- 22:59, 14 July 2008 (diff | hist) Wisconsin: Lignin Project/23 June 2008
- 22:55, 14 July 2008 (diff | hist) Wisconsin: Lignin Project/23 June 2008
- 22:53, 14 July 2008 (diff | hist) Wisconsin: Lignin Project/20 June 2008
- 22:49, 14 July 2008 (diff | hist) Wisconsin: Lignin Project/19 June 2008
- 22:46, 14 July 2008 (diff | hist) Wisconsin: Lignin Project/18 June 2008
- 22:46, 14 July 2008 (diff | hist) Wisconsin: Lignin Project/18 June 2008
- 22:43, 14 July 2008 (diff | hist) Wisconsin: Lignin Project/16 June 2008
- 21:43, 14 July 2008 (diff | hist) N Wisconsin: Lignin Project/27 June 2008 (New page: '''Team Fungus:''' <br> Plate with 1:3 insert to vector ratio had 2 colonies<br> Plate with 0:1 vector had 1 colony<br> Rest had zero colonies<br> We streaked and plated the two colonies f...)
- 21:39, 14 July 2008 (diff | hist) N Wisconsin: Lignin Project/26 June 2008 (New page: '''Team Fungus:''' <br> Performed ligation of insert and vector with ratios of 0:1, 1:0, 1:3, 3:1, and 1:1 <br> Perform transformation of plasmid into E. coli (BL21) and plated to grow ove...)
- 21:28, 14 July 2008 (diff | hist) Wisconsin: Lignin Project/25 June 2008
- 21:27, 14 July 2008 (diff | hist) Wisconsin: Lignin Project/24 June 2008
- 21:27, 14 July 2008 (diff | hist) Wisconsin: Lignin Project/23 June 2008
- 21:27, 14 July 2008 (diff | hist) Wisconsin: Lignin Project/20 June 2008
- 21:27, 14 July 2008 (diff | hist) Wisconsin: Lignin Project/19 June 2008
- 21:26, 14 July 2008 (diff | hist) Wisconsin: Lignin Project/18 June 2008
- 21:26, 14 July 2008 (diff | hist) Wisconsin: Lignin Project/17 June 2008
- 21:26, 14 July 2008 (diff | hist) Wisconsin: Lignin Project/16 June 2008
- 21:25, 14 July 2008 (diff | hist) N Wisconsin: Lignin Project/25 June 2008 (New page: '''Team Fungus:''' <br> Ran digestion of cDNA into pET28a vector<br> Ran gel of digestion and performed gel purification<br>)
- 21:16, 14 July 2008 (diff | hist) Wisconsin: Lignin Project/24 June 2008
- 21:15, 14 July 2008 (diff | hist) Wisconsin: Lignin Project/24 June 2008
- 21:12, 14 July 2008 (diff | hist) N Wisconsin: Lignin Project/24 June 2008 (New page: '''Team Fungus:''' <br> Ran leftovers from yesterday's PCR in 1% agrose gel Used QIAprep miniprep kit to purify pET28a vectors from liquid LB + Kanamycin media that had incubated overnight)
- 21:09, 14 July 2008 (diff | hist) N Wisconsin: Lignin Project/23 June 2008 (New page: '''Team Fungus:''' <br> Ran PCR of Carbon and Nitrogen-limited cDNA<br> Ran PCR products through 0.9% agrose gel at 100V<br>)
- 21:07, 14 July 2008 (diff | hist) Wisconsin: Lignin Project/20 June 2008
- 21:06, 14 July 2008 (diff | hist) Wisconsin: Lignin Project/20 June 2008
- 21:05, 14 July 2008 (diff | hist) N Wisconsin: Lignin Project/20 June 2008 (New page: '''Team Fungus:''' <br> Diluted primers to 100 um, 20 um, and 1 um<br> Made antibiotic stock of CAM<br> Made freezer stock of: pBad24, pBad33, pMCSG7, pET28b (+gene), pET28a, DH1 and BL21<...)
- 20:56, 14 July 2008 (diff | hist) Wisconsin: Lignin Project/19 June 2008
- 20:54, 14 July 2008 (diff | hist) Wisconsin: Lignin Project/19 June 2008
- 20:54, 14 July 2008 (diff | hist) N Wisconsin: Lignin Project/19 June 2008 (New page: '''Team Fungus:''' <br> ---- Took spec reading of Carbon and Nitrogen-limited cDNA<br> Worked on Wiki site<br> Worked on sponsorship<br> Made overnight cultures of: <br> BL21 (x2)<br> pB...)
- 20:47, 14 July 2008 (diff | hist) Wisconsin: Lignin Project/18 June 2008
- 20:46, 14 July 2008 (diff | hist) N Wisconsin: Lignin Project/18 June 2008 (New page: '''Team Fungus:''' <br> Ran Carbon-limited and Nitrogen-limited cDNA on 1% agrose gel<br> -Only ladder showed<br> Analysis: cDNA is single stranded and ethidium bromide dye does bind.)
- 20:40, 14 July 2008 (diff | hist) N Wisconsin: Lignin Project/17 June 2008 (New page: '''Team Fungus:''' <br> Received primers <br> Diluted primers <br> Ran TAQ PCR reaction with Carbon-limited cDNA <br> Ran PCR product on 1% agrose gel; only ladder visible <br>)
- 20:38, 14 July 2008 (diff | hist) N Wisconsin: Lignin Project/16 June 2008 (New page: '''Team Fungus:'''<br> Made LB + Kanamycin plates <br> Streaked out vector (pet28a) on LB + Kanamycin plates <br>)
- 20:32, 14 July 2008 (diff | hist) Team:Wisconsin/Notebook
(Latest | Earliest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)