From 2008.igem.org

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Things we did today

Wetlab work

Antibiotic test

Grace

• Ran HindIII/BamHI digested J33207 on gel
• Extracted from gel
• Ligated with HindIII/BamHI digested pRL1383a
• Used 5 μl ligation reaction to transform 100 μl DH5α MCR cells (from Doug in SC lab)

• Also transformed 25 μl DH5α MCR with 2 μl BB-1 1025 and 1 μl J33207 each.

Checked for Transformants

Krystle

• BB-pRL1383a/K125820 produced a total of 27 transformants of varying size after incubation for 20 hours at 37^oC

Plate of BB-pRL1383a/K125810 transformants under blue light. Only some of the colonies appear to fluoresce and the smaller colonies do not show well in the picture.

Inoculate for Tri-Parental Conjugation

Krystle

• Inoculated 10ml cultures of LB with
  • BB-pRL1383a with sp₁₀₀
  • BB-pRL1383a/K125820 with sp₁₀₀
  • RP1 with kan₅₀

Drylab Work

Project page

Grace

• Wrote up Materials, Methods and Results for Project Part B on team project page.

Sequence analysis

Grace

• Same G->C transversion in CAP binding site for BBpRL-1 sequence.

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

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