Team:Hawaii/Parts

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Contents

Guidelines for naming parts

Part number Part type
K125000-K125099 plasmid
K125100-K125199 promoter
K125200-K125299 ribosome binding site
K125300-K125499 signal sequences, conjugation sequences, random stuff
K125500-K125699 protein coding
K125700-K125799 transcription terminator
K125800-K125999 devices

Parts planned

Parts in process (building)

Part number Description Type
[http://partsregistry.org/wiki/index.php/Part:BBa_K125010 K125010] Broad-Host-Range plasmid derived from RSF1010 plasmid
[http://partsregistry.org/wiki/index.php/Part:BBa_K125330 K125330] P1 lytic replication
[http://partsregistry.org/wiki/index.php/Part:BBa_K125340 K125340] oriV
[http://partsregistry.org/wiki/index.php/Part:BBa_K125510 K125510] Plasmid replication proteins protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125520 K125520] aadA gene protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125800 K125800] rep proteins with promoter and rbs protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125830 K125830] Synechocystis-specific, nitrate-inducible GFP secretion device under the control of a nirA promoter ([http://partsregistry.org/wiki/index.php/Part:BBa_K125100 K125100]+[http://partsregistry.org/wiki/index.php/Part:BBa_B0030 B0030][http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310]+[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500]) device

Parts submitted

Part number Description Type
[http://partsregistry.org/wiki/index.php/Part:BBa_K125000 K125000] pRL1383a derived BioBrick vector containing BioBrick MCS and BBa_J33207 (lac promoter+lacZ) plasmid
[http://partsregistry.org/wiki/index.php/Part:BBa_K125100 K125100] nitrate inducible nir promoter regulator
[http://partsregistry.org/wiki/index.php/Part:BBa_K125110 K125110] nitrate inducible nir promoter + ribosome binding site with efficiency of 0.6 relative to [http://partsregistry.org/wiki/index.php/Part:BBa_B0030 B0030] regulator
[http://partsregistry.org/wiki/index.php/Part:BBa_K125300 K125300] cyanobacterial pilA derived amino termninus protein secretion signal sequence protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310] cyanobacterial slr2016 derived amino terminus protein secretion signal sequence protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500] GFP fusion protein protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125810 K125810] N-terminal slr2016 signal sequence attached to GFP fusion protein (Composite of [http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310]+[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500]) protein coding
[http://partsregistry.org/wiki/index.php/Part:BBa_K125820 K125820] Synechocystis-specific constitutive GFP secretion device under the control of a lac promoter ([http://partsregistry.org/wiki/index.php/Part:BBa_I14032 I14032]+[http://partsregistry.org/wiki/index.php/Part:BBa_B0034 B0034][http://partsregistry.org/wiki/index.php/Part:BBa_K125310 K125310]+[http://partsregistry.org/wiki/index.php/Part:BBa_K125500 K125500]) device
[http://partsregistry.org/wiki/index.php/Part:BBa_K125350 K125350] oriTr/LacZ conjugation screening
[http://partsregistry.org/wiki/index.php/Part:BBa_K125360 K125360] oriT/LacZ conjugation screening
[http://partsregistry.org/wiki/index.php/Part:BBa_K125320 K125320] oriT derived from RP4 origin of transfer
[http://partsregistry.org/wiki/index.php/Part:BBa_K125020 K125020] rep/oriv/aada/orit intermediate for RSF1010 derived plasmid
[http://partsregistry.org/wiki/index.php/Part:BBa_K125530 K125530] aadA gene with promoter and rbs protein coding
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