Recipes

We experimented with several types of growth media for Shewanella growth, both aerobically and anaerobically. The most effective growth media recipes are listed as follows.

Orianna's growth media

• 50 mM K-PIPES 18.925 g/L
• 7.5 mM NaOH 7.5 mL/L
• 28 mM NH₄Cl 1.5 g/L
• 1.3 mM KCl 433 uL/L
• 4.3 mM NaH₂PO₄ 0.59 g/L
• 100 mM NaCl 5.84 g/L
• 18 mM Lactate 1.62 g/L
• 10 mL Vitamin solution
• 10 mL Amino acid solution
• 10 mL Trace mineral solution

LML medium (for anaerobic Shewanella growth)

• 0.02% yeast extract
• 0.01% peptone
• 20 mM lactate
• 10 mM HEPES [pH 7.4]

LML agar (for Shewanella reduction test)

• LML medium
• 0.8% agar
• 2 mM MnO2- Mn(IV) oxide (turns from brown to clear upon reduction to Mn(II))

(LML recipes from [http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9829939| Beliaev and Saffarini 1998])

Chamber media

• 5.844 g/L 100mM NaCl
• 15.1185 g/L 50mM PIPES (hydrogen)

7.0 pH

Phosphate buffer

• 2.918 g/L Monosodium phosphate, monohydrate
• 4.095 g/L Disodium phosphate, anhydrous
• 5.844 g/L 100mM NaCl

7.0 pH

Lactate solution

• 13.51 g/L Lactic acid
• neutralize to pH 7 with NaOH