The TetR system we hoped to develop would have been similar to the lacI system. The pTet promoter which controls mtrB expression is regulated by the repressor TetR ([http://partsregistry.org/wiki/index.php?title=Part:BBa_C0040| BBa_C0040]). Expression of mtrB would have occurred when the system is induced with anhydrotetracycline which binds TetR. Unfortunately, as mtrB is toxic, we could not clone successful clone this system.
However, we did submit parts [http://partsregistry.org/wiki/index.php?title=Part:BBa_K098980 BBa_K098980] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K098981 BBa_K098981] in case you might find them useful.
Light-induced System
We tried to create a light-induced mtrB expression system using the [http://partsregistry.org/Featured_Parts:Light_Sensor light sensor] in the registry. This requires an EnvZ deficient strain of S. oneidensis which we could not successfully generate. Additionally, the registry versions of some of the parts appear wrong (in sequence) or nonfunctional. We thus provide the following two parts for future teams' use:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K098011 E. coli ompR for introduction into other species of bacteria]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K098010 A HO-pcyA protein generator]
"
