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Team:NTU-Singapore/Notebook/13 June 2008
From 2008.igem.org
- Chin Chong
- BL21 cells, containing the LacI-GFP, that were inoculated on thursday were not growing well as they were placed too long in the incubator
- Regrew/Inoculate the BL21 cells at 5pm so that overnight growing of 16hrs would be acheived
- Prepared 20 plates of agar containing Chloranphenicol and 20 plates of agar containing Kanamycin
- Approached Phd students from BIE whom had experience in using the multiplate Fluroescene and luminescene reader
- Varified the correct usage from the student, Zhang Zhengwen, who adviced on some slight modifications to the protocol set for the characterization experiment
- Received the primer designs from 1st Base for: supD, T7ptag and direction checks for T7ptag as well as that for LsrA
