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Things we did today

Wetlab work

Grace

Margaret

This is the gel of the plasmid preps, plus some PCR products of NW and MR.

**Biobrick Plasmid Prep**
Lane	Biobrick/Other	description
1	tri-dye ladder	very light, no difference in intensity
2	B0030	1.75kb, brighter than ladder, a lot of RNA
3	R0010	2.1kb, brighter than ladder, No RNA??
4	C0012	2.5kb, brighter than ladder, a lot of RNA
5	E0040	2.4kb, brighter than ladder, a lot of RNA
6	J33207	2.5kb, maybe 5.7ng/uL, No RNA??
7	rep region	0.5kb, 5.7ng/uL
8	(+) control	bright band below 0.5kb
9	(-) control	all clear
10	SigE	~1kb, maybe 5.7ng/uL

Krystle

name of person/people who performed the task

Summary of task and what was done. Link to experiment for detailed notes if necessary.
e.g. read through papers, worked on proposal, etc.

Use commercial-grade competent cells for E0240 and I14032 extraction? We really need these two Biobricks.

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]