Virginia/8 July 2008

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Goals

  • Prepare overnight broth of single successful colony of Promoter + RBS transformation
  • Plate Screening plasmid (psb1a10) when it arrives from iGem
  • Try again with ligation of RFP and GFP enzyme cut DNA
  • Transform this ligation and pray that it grows in the incubator
  • Run a gel of linearized and cut DNA of BP1 and terminator assembly to verify transformation
  • Begin ligation of standard RBS with BP1 gene to begin parallel assembly of our final operon

Notes

  • Maxiprepped B0015 and B0034 smell strongly of alcohol, we are dubious of the content of the prepared DNA

Accomplished

  • Starting from maixprepped DNA we cut:
    • E0240, I715039, B0034 (dubious, see above), BP1
  • Short Ligations: (45min)
    • Test Vector 1: I715039 + E0240
      • Did a ligation each for non-purified DNA (after digestion) and purified DNA.
    • B0034 (RBS) + BP1
      • Only non-purified post-digestion DNA
    • Plated all short ligations
  • Long Ligations: (overnight @ room temp)
    • Test Vector 1: I715039 + E0240 (unpurified)
    • B0034 (RBS) + BP1
  • Plated screening plasmid [http://partsregistry.org/Part:pSB1A10 pSB1A10] that we received today


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