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Team:Rice University/Notebook/26 June 2008
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- Taylor Stevenson
- Verify function of BleoC biobrick (ptet-rbs-bleoR-stop)
- Transform electro-competent XL1-Blue MRF' cells with the BleoC biobrick.
- Streak transformed cells onto LB/Tet and LB/Tet/Bleo (10ug/mL and 6ug/mL respectively) alongside control cells which have not been transformed.
- Incubate O/N @ 37*C
- Result-Transformed cells showed >100 colonies on both plates while the control cells only grew on the LB/Tet plates. BleoC appears to be functional.
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