Team:Rice University/Notebook/11 July 2008

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  • David Ouyang
    • Digest and Ligate Putative Amber Suppressors
      • Digest Mix
        • 1uL EcoRI
        • 1uL PstI
        • 5uL NEB3
        • 20uL Dilute DNA
        • 1uL BSA
        • 22uL dH2O
      • DCC
      • Ligation Mix
        • 10uL DCCed DNA
        • 1uL T4
        • 2uL Buffer
        • .5uL Vector
        • 7.5 dH2O
  • Taylor Stevenson
    • Prepare 5mL LB O/N of VCS257 (37*C) and VCS257 lambda lysogens (30*C) for preparation of electro-competent cells





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