Team:UNIPV-Pavia/Notebook/Week7
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Week 5: 06/23/08 - 06/27/08
06/30/08
- We received sequencing results for BBa_B0030-BBa_C0061 (4th colony and 7th colony): sequences were correct! We decided to keep the 4th colony.
- Colony PCR for BBa_J23100-BBa_E0240 and BBa_B0030-BBa_C0061: 10 colonies for every plate. Gel showed many working colonies: we chose first colonies for the two ligations.
- We infected 9 ml LB + Amp with 30 µl of:
BBa_C0012 | BBa_B1006 | BBa_R0051-BBa_B0030 | BBa_B0030-BBa_C0061 (4) |
BBa_C0062 | BBa_J23100-BBa_E0240 (1) | BBa_B0030-BBa_C0078 (8) | BBa_J23100-BBa_B0030 |
BBa_C0040 | BBa_I15010 |
07/1/08