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Team:Hawaii/Meeting/2008-07-17
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Contents |
Agenda
- Updates
Minutes
Present: Margaret, Krystle, Grace, Norman, SC, LG, GP Advice
- Transformations: don't go over 10% of DNA added to competent cells, this dilutes them too much.
- For the pRL1383a construct, use the omega interposon, not just the aadA gene. The former is a complete package already.
Action Items
- Krystle and Grace:
- e-mail headquarters to ask for the BioBrick parts you need. Make sure to tell them about all of your successes so they know you are credible.
- May also be worth trying to PCR amplify the biobrick parts you need.
- Verify your emergency pRL1383a with the primers for your insert.
- Restriction Enzymes
- Check a few different catalogues (Roche, Promega, etc.) to see if they have buffers which are compatible with multiple enzymes running simultaneously.
- Separately cut the construct with enzymes. See which one makes the ladder. Let's try to figure out why this ladder is happening and how to avoid getting it.
- Lichenase
- Order the genomic DNA containing the lichenase gene.
- For a back-up, we can ask Dr. Schara if she has any interesting enzymes we could use. The only problem is that by not using Lichenase, we lose a positive control and there will be no way to tie it into the literature.
Coming Up
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
