Team:Hawaii/Notebook/2008-07-17

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Things we did today

Wetlab work

PCR

Grace

Colony PCR for GFP fusion brick and new pRL1383a to verify inserts
Sequencing (25 mu;l reactions)

nir promoter
slr2016
pilA
BBa_B0015
BBa_B0024
BBa_B0030
BBa_C0012
BBa_E0040
BBa_J04430
BBa_J33207
BBa_R0010

Ran all PCR products on a 4% gel to check

Gel ran funny -- loading dye ran 2x as fast as the DNA itself
Poor ladder resolution on gel
No bands seen for GFP fusion, pRL1383a, R0010
Incorrect bands for B0015 and J33207
Will redo plasmid preps for R0010, B0015, J33207
Will redo PCR reaction tomorrow for pRL1383a, GFP fusion, B0015, J33207, R0010

Plasmid Prep

Grace

Inoculated 7 ml LB + amp₁₀₀ with single colony of B0015, R0010, J04430 for redo of plasmid prep tomorrow

Restreak to Purify

Grace

Restreaked GFP fusion and pRL1383a (MCS replaced) transformants to purify

pSB3K3 plasmid prep

Margaret

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

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