Team:Hawaii/Protocols/ExoSAP

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Overview

ExoSAP removes ssDNA and dNTPs

Protocol

1. Add 2 μl ExoSAP to 5 μl of the PCR reaction. 2. Incubate in a 37C water bath for 15 min. 3. Incubate in a 80C water bath for 15 min. to inactivate ExoSAP.

Retrieved from "http://2008.igem.org/Team:Hawaii/Protocols/ExoSAP"