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Team:Hawaii/Project
From 2008.igem.org
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Overall Project
Cyanobacteria are frequently studied for their ability to harness the power of photosynthesis in order to produce a wide variety of useful products including bio-fuels and -polymers. Such tasks are accomplished by these ‘little green factories’ with a minimal input of salts, light, and carbon dioxide. We aim to expand the availability of BioBrick vectors to cyanobacteria in order to “open source” the current BioBrick registry to a greater range of organisms.
We plan to engineer:
- 1) a mobilizable broad-host-range BioBrick vector that can be used to transfer genetic information between E. coli and Synechocystis sp. 6803, with the future possibility of transforming plants via Agrobacteria;
- 2) a cassette for protein export from Synechocystis; and
- 3) the nitrate-inducible cyanobacterial nir promoter.
The functionality of the parts we engineer will be demonstrated by achieving inducible protein production and export of a lichenase and a GFP construct introduced into Synechocystis using our novel BioBrick shuttle vector.
Project Details
- Part A: Mobilizable Broad-Host-Range Plasmid
- Part B: Cyanobacterial protein secretion system
Results
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
